BUTYRYLCHOLINESTERASE IN HUMAN-BRAIN AND ACETYLCHOLINESTERASE IN HUMAN-PLASMA - TRACE ENZYMES MEASURED BY 2-SITE IMMUNOASSAY
BUTYRYLCHOLINESTERASE IN HUMAN-BRAIN AND ACETYLCHOLINESTERASE IN HUMAN-PLASMA - TRACE ENZYMES MEASURED BY 2-SITE IMMUNOASSAY
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DOI：
10.1111/j.1471-4159.1988.tb03091.x
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发表时间：
1988-10-01
影响因子：
4.7
通讯作者：
HAMMOND, P
中科院分区：
文献类型：
--
作者：
BRIMIJOIN, S;HAMMOND, P
乙酰胆碱酯酶 (AChE) 和丁酰胆碱酯酶 (BuChE) 的酶联免疫吸附测定明显比使用选择性酶抑制剂的传统测定更具特异性。新的检测方法用于血液和大脑样本，其中含有微量的一种酶，而另一种酶则占主导地位。结果显示，人血浆中确实含有AChE（8 ng/ml），尽管其主要胆碱酯酶是BuChE（3,300 ng/ml）。在人红细胞中未检测到BuChE免疫反应性，但在所有大脑区域都存在。小脑是测试最丰富的区域（540 ng BuChE/g组织），而大脑皮层是最差的 （240 ng BuChE/g）。然而，由于局部 AChE 含量较低（99 ng/g），BuChE 是主要的皮质胆碱酯酶。壳核中的情况则相反，BuChE 免疫反应性 (340 ng/g) 远远超过 AChE (6,100 ng/g)。
Enzyme-linked immunosorbent assays for acetylcholinesterase (AChE) and for butyrylcholinesterase (BuChE) were markedly more specific than conventional assays using selective enzyme inhibitors. The new assays were used with blood and brain samples containing traces of one enzyme dominated by large amounts of the other. The results showed that human plasma does contain AChE (8 ng/ml), even though its major cholinesterase is BuChE (3,300 ng/ml. BuChE immunoreactivity was not detected in human red blood cells but occurred in all brain regions. The cerebellum was the richest region tested (540 ng of BuChE/g of tissue), whereas the cerebral cortex was the poorest (240 ng of BuChE/g). However, because of the small local AChE content (99 ng/g), BuChE was the major cortical cholinesterase. The picture was reversed in the putamen, where BuChE immunoreactivity (340 ng/g) was far outweighed by that of AChE (6,100 ng/g).