Sideroxyarcus emersonii gen. nov. sp. nov., a neutrophilic, microaerobic iron- and thiosulfate-oxidizing bacterium isolated from iron-rich wetland sediment
Sideroxyarcus emersonii gen. nov. sp. nov., a neutrophilic, microaerobic iron- and thiosulfate-oxidizing bacterium isolated from iron-rich wetland sediment
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Sideroxyarcus emersonii gen.
DOI：
10.1099/ijsem.0.005347
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发表时间：
2022
影响因子：
2.8
通讯作者：
Ohkuma Moriya
中科院分区：
文献类型：
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作者：
Kato Shingo;Itoh Takashi;Iino Takao;Ohkuma Moriya
对先前从日本茨城市湿地分离到的一株中性嗜铁氧化细菌MIZ01T进行了详细的分类鉴定。菌株miz01t是一种可运动的弯曲棒状革兰氏阴性细菌。它能在10-40°C（30-35°C）和pH 5.5-7.0 （pH 6.0）下生长。除了亚铁外，它还以硫代硫酸盐作为唯一的电子供体进行微生物和化学岩石自养生长。菌株miz01t的主要细胞脂肪酸为C16: 1ω7c/C16: 1ω6c和C16: 0。测定其全基因组序列（2.74 Mbp）， DNA G+C含量为60.0 mol%。系统发育分析表明，菌株miz01t属于β变形菌门gallionellaceae，与暂定名为“Sideroxydans lithotrophicus”ES-1的分离株亲缘关系密切（16S rRNA基因序列相似性为98.2%）。根据该菌株的表型和系统发育特征，我们认为该菌株miz01代表了gallionellaceae科的一个新属和新种，我们建议将其命名为esideroxyarcus emersoniigen。类型应变为应变MIZ01T（=JCM 39089T=DSM 111897T）。
A neutrophilic iron-oxidizing bacterium, strain MIZ01T, which was previously isolated from a wetland in Ibaraki, Japan, was taxonomically characterized in detail. Strain MIZ01Twas a motile, curved-rod shaped, Gram-stain-negative bacterium. It was able to grow at 10–40 °C (optimally at 30–35 °C) and at pH 5.5–7.0 (optimally at pH 6.0). It grew microaerobically and chemolithoautotrophically using thiosulfate, in addition to ferrous iron, as the sole electron donor. Major cellular fatty acids of strain MIZ01Twere C16 : 1ω7c/C16 : 1ω6cand C16 : 0. The complete genome sequence (2.74 Mbp) was determined, showing that its DNA G+C content was 60.0 mol%. Phylogenetic analyses indicated that strain MIZ01Tbelonged to the familyGallionellaceae, classBetaproteobacteria, and was closely related to an isolate tentatively named ‘Sideroxydans lithotrophicus’ ES-1 (98.2 % of 16S rRNA gene sequence similarity). Based on its phenotypic and phylogenetic characteristics, we conclude that strain MIZ01Trepresents a new genus and species in the familyGallionellaceaefor which we propose the nameSideroxyarcus emersoniigen. nov., sp. nov. The type strain is strain MIZ01T(=JCM 39089T=DSM 111897T).