Decapentaplegic function in wing vein development and wing morph transformation in brown planthopper, Nilaparvata lugens
Decapentaplegic function in wing vein development and wing morph transformation in brown planthopper, Nilaparvata lugens
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褐飞虱翅脉发育和翅形态转化中的十肢瘫痪功能
DOI：
10.1016/j.ydbio.2019.02.016
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发表时间：
2019-05-15
影响因子：
2.7
通讯作者：
Hua, Hongxia
中科院分区：
文献类型：
--
作者：
Li, Xiang;Liu, Fangzhou;Hua, Hongxia
十指肠麻痹(DPP)基因在多种细胞和分子的生长发育过程中发挥着重要作用。在昆虫中，DPP主要用于背腹纹饰和附肢的形成。褐飞虱(BPH)褐飞虱是一种主要的水稻害虫，具有两种不同的翅型，分别为长翅型和短翅型。以本实验室稳定的褐飞虱LW和SW BPH品系为材料，利用RNA干扰(RNAi)技术研究了褐飞虱DPP(Nldpp)在羽翼发育中的作用。在西南品系中，Nldpp的沉默导致前翼显著延长，而在LW品系中，Nldpp-敲除导致翅膀扭曲。此外，Nldpp的击倒导致完全没有机翼静脉。在翼垫发育过程中，西南品系Terga的Nldpp丰度显著高于LW品系。通过控制翅膀形态转化的方向，我们发现在NlInR1基因敲除的BPH中，Nldpp的表达水平增加(倾向于Sw)，而Nldpp的丰度降低(倾向于LW)。我们的结果表明Nldpp在BPH的静脉形成和发育中起主要作用。此外，Nldpp还可受NlInR1/2调控，参与翅膀的形态转化。
The decapentaplegic (dpp) gene plays a variety of roles in diverse cellular and molecular processes of the growth and development. In insects, dpp is mainly required for dorsal-ventral patterning and appendage formation. The brown planthopper (BPH) Nilaparvata lugens, a major pest of rice, possesses two distinct wing morphs described as long-winged (LW) and short-winged (SW) morphs. With our lab-maintained stable strains of LW and SW BPH, RNA interference (RNAi) was used to research the functions of N. lugens dpp (Nldpp) on wing development. Silencing of Nldpp in the SW strain led to the significant lengthening of the forewing, while Nldpp-knockdown in the LW strain resulted in distorted wings. Moreover, knockdown of Nldpp caused the complete absence of wing veins. During the development of wing-pads, the Nldpp abundance in the terga of the SW strain was significantly higher than that of the LW strain. Through controlling the direction of wing morph transformation, we found that the expression level of Nldpp increased in the NlInR1-knockdown BPH (tending to SW) and abundance of Nldpp declined after dsNiInR2 injection (tending to LW). Our results showed that Nldpp is mainly responsible for the formation and development of veins in BPH. Also, Nldpp can be regulated by NlInR1/2 and participate in the wing morph transformation.