BcMF11, a novel non-coding RNA gene from Brassica campestris, is required for pollen development and male fertility
BcMF11, a novel non-coding RNA gene from Brassica campestris, is required for pollen development and male fertility
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BcMF11 是一种来自甘蓝的新型非编码 RNA 基因，是花粉发育和雄性生育能力所必需的
DOI：
10.1007/s00299-012-1337-6
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发表时间：
2013-01-01
影响因子：
6.2
通讯作者：
Wang, Cheng-Gang
中科院分区：
文献类型：
--
作者：
Song, Jiang-Hua;Cao, Jia-Shu;Wang, Cheng-Gang
关键信息BcMF11是一个非编码RNA基因，在花粉发育过程中起重要作用，利用反义RNA技术可能对调控作物花粉育性有重要意义。SSP. chinensis Makino）。然而，关于BcMF 11在花粉发育中的功能知之甚少。为了研究BcMF 11在花粉发育中的生物学作用，在绒毡层特异性启动子BcA9和组成型启动子CaMV 35S的控制下，对BcMF 11 cDNA进行了反义抑制。反义RNA转基因植株表现出BcMF11表达的下降，并表现出明显的形态缺陷。花粉离体和体内萌发试验表明，抑制BcMF 11基因表达会降低花粉萌发率，延缓花粉管在花柱中的伸长。扫描电镜下观察到转反义BcMF11基因大白菜花粉粒皱缩、塌陷。进一步的细胞学观察发现，转基因植株花粉发育过程异常，包括绒毡层延迟降解、小孢子不同步分离、花粉粒发育失败等。这些结果表明，BcMF11作为一种非编码RNA，在花粉发育和雄性育性中起着至关重要的作用。
Key messageBcMF11as a non-coding RNA genehas an essential role in pollen development, and might be useful for regulating the pollen fertility of crops by antisense RNA technology.AbstractWe previously identified a 828-bp full-length cDNA of BcMF11, a novel pollen-specific non-coding mRNA-like gene from Chinese cabbage (Brassica campestris L. ssp. chinensis Makino). However, little information is known about the function of BcMF11 in pollen development. To investigate its exact biological roles in pollen development, the BcMF11 cDNA was antisense inhibited in transgenic Chinese cabbage under the control of a tapetum-specific promoter BcA9 and a constitutive promoter CaMV 35S. Antisense RNA transgenic plants displayed decreasing expression of BcMF11 and showed distinct morphological defects. Pollen germination test in vitro and in vivo of the transgenic plants suggested that inhibition of BcMF11 decreased pollen germination efficiency and delayed the pollen tubes’ extension in the style. Under scanning electron microscopy, many shrunken and collapsed pollen grains were detected in the antisense BcMF11 transgenic Chinese cabbage. Further cytological observation revealed abnormal pollen development process in transgenic plants, including delayed degradation of tapetum, asynchronous separation of microspore, and aborted development of pollen grain. These results suggest that BcMF11, as a non-coding RNA, plays an essential role in pollen development and male fertility.