Microarray screening and qRT-PCR evaluation of microRNA markers for forensic body fluid identification
Microarray screening and qRT-PCR evaluation of microRNA markers for forensic body fluid identification
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DOI：
10.1002/elps.201400075
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发表时间：
2014-11-01
期刊：
影响因子：
2.9
通讯作者：
Kim, Seon-Young
中科院分区：
文献类型：
--
作者：
Park, Jong-Lyul;Park, Seong-Min;Kim, Seon-Young
MicroRNA（miRNA）是一类在转录后水平调控多种生物学过程的小分子非编码RNA。miRNAs在法医体液鉴定中具有巨大的潜力，因为它们以组织特异性方式表达并且不易降解。先前的研究报道了几种体液特异性miRNA，但它们之间很少有重叠。在这里，我们使用了一个全基因组的miRNA微阵列包含超过1700个miRNA检测20个体液样本，并确定新的miRNA用于法医体液鉴定。基于Shannon熵和Q统计量，首先选择在每种体液中特异性表达的203个miRNAs。然后选择八种miRNA作为新的法医学相关miRNA标记物：血液的miR-484和miR-182，唾液的miR-223和miR-145，精液的miR-2392和miR-3197，以及阴道分泌物的miR-1260 b和miR-654- 5 p。当通过qRT-PCR在另外40个体液样品中评估所选择的8种miRNA时，它们显示出用于鉴定靶体液的高灵敏度和特异性。我们认为这八种miRNAs可能是开发一种有效的法医体液鉴定分子检测方法的候选者。
MicroRNAs (miRNA) are a class of small (similar to 22 nucleotides) noncoding RNAs that regulate diverse biological processes at the post-transcriptional level. MiRNAs have great potential for forensic body fluid identification because they are expressed in a tissue specific manner and are less prone to degradation. Previous studies reported several miRNAs as body fluid specific, but there are few overlaps among them. Here, we used a genome-wide miRNA microarray containing over 1700 miRNAs to assay 20 body fluid samples and identify novel miRNAs useful for forensic body fluid identification. Based on Shannon Entropy and Q-statistics, 203 miRNAs specifically expressed in each body fluid were first selected. Eight miRNAs were then selected as novel forensically relevant miRNA markers: miR484 and miR-182 for blood, miR-223 and miR-145 for saliva, miR-2392 and miR-3197 for semen, and miR-1260b and miR-654-5p for vaginal secretions. When the eight selected miRNAs were evaluated in 40 additional body fluid samples by qRT-PCR, they showed high sensitivity and specificity for the identification of the target body fluid. We suggest that the eight miRNAs may be candidates for developing an effective molecular assay for forensic body fluid identification.