Studies of nutrition and autoimmunity. Failure of zinc deprivation to alter autoantibody production when initiated in disease-established mice.
Studies of nutrition and autoimmunity. Failure of zinc deprivation to alter autoantibody production when initiated in disease-established mice.
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DOI：
10.1093/jn/117.1.177
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
K. Vruwink;C. Keen;M. Gershwin;L. Hurley
中科院分区：
文献类型：
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作者：
K. Vruwink;C. Keen;M. Gershwin;L. Hurley
早期的研究表明，在年轻的NZB小鼠中开始缺锌时，可以延缓自身免疫的发展，并导致寿命延长。目前的研究评估了锌剥夺在患有疾病的NZB小鼠中的可能益处，因为该模型与人类患者更相关。6-8月龄雌性NZB小鼠饲喂含80 μ g Zn/g和10 μ g Cu/g（对照）或1 μ g Zn/g和10 μ g Cu/g（缺锌）的饲料4个月。此外，另一组被喂食含有1微克Zn/g和100微克Cu/g的饮食，以确定是否锌缺乏会因高饮食铜通过铜与锌在肠道水平的竞争而加剧（锌缺乏+高铜）。第四组小鼠以与缺锌组相同的摄入量喂食对照饮食，以控制与缺锌相关的饥饿（限制摄入）。无论饮食治疗，所有小鼠以相同的速度产生抗红细胞抗体。在4个月结束时，82%的对照组和限制摄入组的存活率，而缺锌组的存活率为38%，缺锌+高铜组的存活率为50%。这些观察结果表明，与年轻NZB小鼠的发现相反，具有已建立的自身免疫性的成年NZB小鼠的锌剥夺不会改善存活率。事实上，严重缺锌会增加死亡率，这表明需要考虑极端饮食的潜在危害。
Earlier studies have demonstrated that zinc deprivation, when begun in young NZB mice, can retard the development of autoimmunity and result in an increased life span. The present study evaluated the possible benefits of zinc deprivation in NZB mice with established disease, as this model is more relevant to the human patient. Female NZB mice aged 6-8 mo were fed diets containing either 80 micrograms Zn/g and 10 micrograms Cu/g (control) or 1 microgram Zn/g and 10 micrograms Cu/g (zinc-deficient) for 4 mo. In addition, another group was fed a diet containing 1 microgram Zn/g and 100 micrograms Cu/g to determine whether zinc deficiency could be exacerbated by high dietary copper through a competition of copper with zinc at the intestinal level (zinc deficient + high copper). A fourth group of mice was fed the control diet at the same intake as that of the zinc-deficient group in order to control for the inanition associated with zinc deficiency (restricted intake). Regardless of dietary treatment, all mice developed antierythrocyte antibodies at the same rate. At the end of 4 mo, 82% of the control and the restricted-intake groups had survived, whereas the zinc-deficient group had a 38% survival rate and the zinc-deficient + high copper group had a 50% survival rate. These observations show that, in contrast to findings with younger NZB mice, zinc deprivation of adult NZB mice with established autoimmunity will not improve survival. Indeed, severe zinc deficiency increased the mortality rate, demonstrating the need to consider the potential hazards of dietary extremes.