Proteomics analysis of starved cells revealed Annexin A1 as an important regulator of autophagic degradation.
Proteomics analysis of starved cells revealed Annexin A1 as an important regulator of autophagic degradation.
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DOI：
10.1016/j.bbrc.2011.03.067
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发表时间：
2011-04-15
影响因子：
3.1
通讯作者：
Yin XM
中科院分区：
文献类型：
--
作者：
Kang JH;Li M;Chen X;Yin XM
大自噬参与了长寿命胞质蛋白和亚细胞器的大量降解，这对细胞在饥饿期间的存活至关重要。为了鉴定自噬过程的潜在参与者，我们对在完全培养基和Earle平衡盐溶液中培养的HCT 116细胞进行蛋白质组学分析。在检测到的大约1,500个蛋白质点中，我们鉴定了52个独特的蛋白质，其表达水平在饥饿后发生了显著变化。值得注意的是，我们发现膜联蛋白A1在mRNA和蛋白水平上在饥饿后显著上调。用特异性siRNA抑制膜联蛋白A1表达并不改变饥饿诱导的自噬，如通过脂化LC 3水平所测量的，但显著逆转自噬降解，如通过p62/SQSTM 1水平所测量的。因此，膜联蛋白A1似乎是积极上调饥饿期间，以促进自噬降解。总之，本研究中提供的数据建立了饥饿细胞中蛋白质组的表达谱，这使得能够鉴定在饥饿诱导的自噬中具有潜在意义的蛋白质。
Macroautophagy is involved in the bulk degradation of long-lived cytosolic proteins and subcellular organelles, which is important for the survival of cells during starvation. To identify potential players of the autophagy process, we subjected HCT116 cells cultured in complete medium and in Earle's balanced salt solution to proteomics analysis. In approximately 1,500 protein spots detected, we characterized 52 unique proteins, whose expression levels were significantly changed following starvation. Notably, we found that Annexin A1 was significantly upregulated following starvation at both mRNA and protein levels. Inhibition of Annexin A1 expression with specific siRNA did not alter starvation-induced autophagy as measured by the level of lipidated LC3, but significantly reversed autophagy degradation as measured by the level of p62/SQSTM 1. Thus Annexin A1 seemed to be positively upregulated during starvation to promote autophagic degradation. Overall, the data presented in this study established a expression profile of the proteome in starved cells, which allowed the identification of proteins with potential signficance in starvation-induced autophagy.
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影响因子：
4.4
作者：
Kang, Jeong Han;Park, Kwan-Kyu;Chang, Young-Chae
通讯作者：
Chang, Young-Chae
影响因子：
4.8
作者：
Ding, Wen-Xing;Ni, Hong-Min;Yin, Xiao-Ming
通讯作者：
Yin, Xiao-Ming
DOI：
10.1016/j.bbrc.2008.01.112
发表时间：
2008-04-11
影响因子：
3.1
作者：
Matsumoto, Naomi;Ezaki, Junji;Kominami, Eiki
通讯作者：
Kominami, Eiki
p62/SQSTM1形成自噬降解的蛋白质聚集体，并对亨廷顿蛋白诱导的细胞死亡具有保护作用。
影响因子：
7.8
作者：
Bjorkoy, Geir;Lamark, Trond;Brech, Andreas;Outzen, Heidi;Perander, Maria;Overvatn, Aud;Stenmark, Harald;Johansen, Terje
通讯作者：
Johansen, Terje
影响因子：
7
作者：
Kristensen, Anders Riis;Schandorff, Soren;Andersen, Jens S.
通讯作者：
Andersen, Jens S.