MicroRNA-4458 suppresses migration and epithelial-mesenchymal transition via targeting HMGA1 in non-small-cell lung cancer cells
MicroRNA-4458 suppresses migration and epithelial-mesenchymal transition via targeting HMGA1 in non-small-cell lung cancer cells
复制标题
MicroRNA-4458 通过靶向 HMGA1 抑制非小细胞肺癌细胞的迁移和上皮间质转化
DOI：
10.2147/cmar.s185117
复制
发表时间：
2019-01-01
影响因子：
3.3
通讯作者：
Li, Yaming
中科院分区：
文献类型：
--
作者：
Ma, Yu;Li, Xuena;Li, Yaming
目的：越来越多的研究表明microRNA-4458（miR-4458）与人类肿瘤进展相关。然而，miR-4458在非小细胞肺癌（NSCLC）中的分子机制仍不清楚。本研究旨在揭示miR-4458在NSCLC中的生物学功能。材料与方法：采用qRT-PCR方法检测miR-4458在NSCLC细胞中的表达。转染后进行体外细胞增殖和迁移实验。结果：miR-4458在NSCLC细胞中表达明显下调。miR-4458的过表达强烈降低NSCLC细胞系中的增殖和迁移。此外，miR-4458通过PI 3 K/AKT途径抑制迁移和上皮-间充质转化（EMT）的进展。结论：miR-4458参与了NSCLC的迁移过程，通过直接靶向HMGA 1实现EMT，miR-4458可能成为NSCLC治疗的新靶点。
Purpose: Increasing studies have shown that microRNA-4458 (miR-4458) is associated with human cancer progression. However, the molecular mechanism of miR-4458 in non-small-cell lung cancer (NSCLC) remains largely unknown. This study aims to reveal the biological function of miR-4458 in NSCLC.Materials and methods: The expression of miR-4458 in NSCLC cells was evaluated by qRT-PCR. Cell proliferation and migration assay were carried out in vitro after transfection. A luciferase reporter and Western blot assay were performed to identify the functional target of miR-4458.Results: The study indicated that miR-4458 was markedly downregulated in NSCLC cells. Overexpression of miR-4458 strongly reduced the proliferation and migration in NSCLC cell lines. In addition, miR-4458 inhibited the progression of migration and epithelial-mesenchymal transition (EMT) through the PI3K/AKT pathway. Luciferase report assay demonstrated that HMGA1 was a target gene for miR-4458.Conclusion: The results indicate that miR-4458 participated in the process of migration and EMT via directly targeting HMGA1 and miR-4458 might be a potential novel therapeutic target in NSCLC.