Characterization of murine middle repetitive DNA.
Characterization of murine middle repetitive DNA.
复制标题
小鼠中间重复 DNA 的表征。
DOI：
10.1089/dna.1993.12.319
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发表时间：
1993
影响因子：
3.1
通讯作者：
Woodworth,ME
中科院分区：
文献类型：
--
作者：
Adiletta,DC;Elliott,RW;Woodworth,ME
我们从一个包含小鼠重复家族子集的小文库中鉴定了四个序列。每个克隆的每个基因组的重复频率低于 1,100 个拷贝，并且每个克隆代表一个独特的中间重复 DNA 家族。一个克隆 (pMR111) 与小鼠脑池内 A 颗粒 (IAP) 元件具有同源性，第二个克隆 (pMR89) 与人纤维蛋白基因 3' 非翻译区的序列具有部分同源性，而两个克隆 (pMR6、pMR66) 是新序列，与任何报道的 DNA 序列没有同源性。每个克隆与来自小鼠一种或多种组织的一种或两种离散的RNA转录物杂交。克隆 pMR66 在小鼠基因组 DNA 的三个基因座上检测到限制性片段长度多态性 (RFLP)，定义了 5 号染色体远端和 7 号染色体近端的基因座。第三个基因座未作图谱。这项研究表明，重复 DNA 文库中的克隆重复序列是遗传标记的潜在来源。
We have characterized four sequences from a small library containing a subset of the repetitive families of the mouse. Each clone has a repetition frequency of less than 1,100 copies per genome and each clone represents a unique family of middle repetitive DNA. One clone (pMR111) shares homology with mouse intracisternal A-particle (IAP) elements, a second clone (pMR89) has partial homology with a sequence in the 3′ untranslated region of the human fibulin gene, while two clones (pMR6, pMR66) are new sequences that have no homology to any reported DNA sequence. Each clone hybridizes to one or two discrete RNA transcripts from one or more tissues of the mouse. Clone pMR66 detected restriction fragment length polymorphisms (RFLPs) at three loci in genomic mouse DNA, defining loci at the distal end of chromosome 5 and the proximal end of chromosome 7. The third locus is unmapped. This study demonstrates that cloned repeats from a repetitive DNA library are a potential source of genetic markers.