Reconstructing a hydrogen-driven microbial metabolic network in Opalinus Clay rock.
Reconstructing a hydrogen-driven microbial metabolic network in Opalinus Clay rock.
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DOI:
10.1038/ncomms12770
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发表时间:
2016-10-14
影响因子:
16.6
通讯作者:
Bernier-Latmani, Rizlan
中科院分区:
文献类型:
--
作者:
Bagnoud, Alexandre;Chourey, Karuna;Hettich, Robert L.;de Bruijn, Ino;Andersson, Anders F.;Leupin, Olivier X.;Schwyn, Bernhard;Bernier-Latmani, Rizlan
The Opalinus Clay formation will host geological nuclear waste repositories in Switzerland. It is expected that gas pressure will build-up due to hydrogen production from steel corrosion, jeopardizing the integrity of the engineered barriers. In an in situ experiment located in the Mont Terri Underground Rock Laboratory, we demonstrate that hydrogen is consumed by microorganisms, fuelling a microbial community. Metagenomic binning and metaproteomic analysis of this deep subsurface community reveals a carbon cycle driven by autotrophic hydrogen oxidizers belonging to novel genera. Necromass is then processed by fermenters, followed by complete oxidation to carbon dioxide by heterotrophic sulfate-reducing bacteria, which closes the cycle. This microbial metabolic web can be integrated in the design of geological repositories to reduce pressure build-up. This study shows that Opalinus Clay harbours the potential for chemolithoautotrophic-based system, and provides a model of microbial carbon cycle in deep subsurface environments where hydrogen and sulfate are present. Hydrogen build-up in geological nuclear waste repositories poses risks, but it may be alleviated by H2 consumption by deep subsurface microbial communities. Here, the authors inject H2 in a borehole and use metagenomics and metaproteomics to identify a carbon cycle driven by autotrophic H2 oxidizers.
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影响因子:
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通讯作者:
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