Differences in systemic and central nervous system cellular immunity relevant to relapsing-remitting multiple sclerosis
Differences in systemic and central nervous system cellular immunity relevant to relapsing-remitting multiple sclerosis
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DOI：
10.1007/s00415-005-0778-z
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发表时间：
2005-08-01
影响因子：
6
通讯作者：
Saida, T
中科院分区：
文献类型：
--
作者：
Matsui, M;Araya, S;Saida, T
为了阐明与多发性硬化症（MS）恶化相关的全身和中枢神经系统（CNS）免疫之间的差异，我们同时使用流式细胞术检测36例复发缓解型MS （RRMS）未治疗患者的外周血和脑脊液（CSF）样本，以确定功能性淋巴细胞亚群的百分比，并使用酶联免疫吸附法测定可溶性免疫介质。活跃的RRMS患者（n = 27）的特点是血液中CD4(+) CXCR3(+) Th1细胞比不活跃的患者（n = 9）增加，该参数与血浆中IL-10和IL-12p70水平呈负相关。相比之下，CD4(+)CD25（+）细胞百分比的增加和CD8(+) CD11a（高）细胞百分比的减少是来自活性RRMS的CSF样本的特征。此外，CSF CD4(+) CD25（+）细胞与CSF中白细胞计数以及白蛋白和CXCL10水平密切相关，因此，可以作为CNS炎症反应的测量指标。另一方面，CD8(+)CD11a（高）细胞可能具有免疫调节细胞的功能，因为它们在脑脊液中的百分比与脑脊液中抗炎细胞因子IL-4的水平呈正相关。这些研究结果表明，MS复发与外周血和脑脊液间不同的细胞介导免疫改变相结合，而淋巴细胞亚群的测量可能有助于监测疾病状态。
In order to elucidate the differences between systemic and central nervous system (CNS) immunity that are relevant to exacerbations of multiple sclerosis ( MS), paired peripheral blood and cerebrospinal fluid (CSF) samples obtained from 36 non-treated patients with relapsing-remitting MS (RRMS) were simultaneously examined using flow cytometry to determine the percentages of functional lymphocyte subsets, as well as enzyme-linked immunosorbent assays for measuring soluble immune mediators. Active RRMS patients (n = 27) were characterized by an increase in CD4(+) CXCR3(+) Th1 cells in blood as compared with inactive patients (n = 9), and this parameter was inversely correlated with plasma levels of IL-10 and IL-12p70. In contrast, an increase in the percentage of CD4(+)CD25(+) cells and a decrease in the percentage of CD8(+) CD11a(high) cells were features of CSF samples from those with active RRMS. Further, CSF CD4(+) CD25(+) cells had a close association with leukocyte counts as well as albumin and CXCL10 levels in the CSF, and, thus, could be useful as a measure for inflammatory reactions in the CNS. On the other hand, CD8(+)CD11a(high) cells may function as immunoregulatory cells, as their percentage in the CSF showed a positive correlation with CSF levels of the anti-inflammatory cytokine IL-4. These findings suggest that MS relapses occur in a combination with altered cell-mediated immunity that differs between the peripheral blood and CSF compartments, while measurement of lymphocyte subsets may be helpful for monitoring disease status.