Acute effects of 1-methyl-4-phenylpyridinium ion (MPP+) on dopamine and serotonin metabolism in rat striatum as assayed in vivo by a micro-dialysis technique
Acute effects of 1-methyl-4-phenylpyridinium ion (MPP+) on dopamine and serotonin metabolism in rat striatum as assayed in vivo by a micro-dialysis technique
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通过微透析技术体内测定 1-甲基-4-苯基吡啶鎓离子 (MPP ) 对大鼠纹状体多巴胺和血清素代谢的急性影响
DOI：
10.1007/bf01253601
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发表时间：
2005
影响因子：
3.3
通讯作者：
T. Nagatsu
中科院分区：
文献类型：
--
作者：
N. Ozaki;D. Nakahara;N. Kaneda;K. Kiuchi;T. Okada;Y. Kasahara;T. Nagatsu
通过体内微透析技术检查了 1-甲基-4-苯基吡啶鎓离子 (MPP+)（一种源自 1-甲基-4-苯基-1,2,3,6-四氢吡啶 (MPTP) 的神经毒素）的急性作用。将透析插管植入大鼠纹状体，采用高效液相色谱电化学检测法（HPLC-ED）测定MPP+给药后每20 min灌注液中多巴胺（DA）、3,4-二羟基苯乙酸（DOPAC）和高香草酸（HVA）以及5-羟基吲哚乙酸（5-HIAA）浓度的变化。 MPP+给药后，DOPAC、HVA和5-HIAA的水平显着降低。相反，DA水平显着增加，并在MPP+给药开始后40分钟达到最大值。通过对纹状体组织的死后分析，证明MPP+会抑制单胺氧化酶(MAO)，尤其是MAO-B。这些结果表明MPP+在体内引起的急性生化变化是MAO抑制和DA释放。
The acute effect of 1-methyl-4-phenylpyridinium ion (MPP+), a neurotoxin derived from 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP), was examined by the in vivo micro-dialysis technique. A dialysis cannula was implanted into rat striatum, and the changes in the concentrations of dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA), and 5-hydroxyindoleacetic acid (5-HIAA) in the perfusate every 20 min after administration of MPP+were determined by high-performance liquid chromatography with electrochemical detection (HPLC-ED). After MPP+administration the levels of DOPAC, HVA and 5-HIAA were markedly decreased. On the contrary the level of DA was markedly increased and reached a maximum 40 min after beginning of the MPP+administration. By postmortem analysis of the striatal tissue MPP+was proved to cause the inhibition of monoamine oxidase (MAO), especially MAO-B. These results suggest that the acute biochemical changes induced by MPP+in vivo were MAO inhibition and release of DA.