Sensitive Detection of Nitrite and Nitrate in Seawater by 222 nm UV-Irradiated Photochemical Conversion to Peroxynitrite and Ion Chromatography-Luminol Chemiluminescence System
Sensitive Detection of Nitrite and Nitrate in Seawater by 222 nm UV-Irradiated Photochemical Conversion to Peroxynitrite and Ion Chromatography-Luminol Chemiluminescence System
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DOI：
10.1021/acs.est.3c00273
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发表时间：
2023-03-27
影响因子：
11.4
通讯作者：
Tomiyasu, Takashi
中科院分区：
文献类型：
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作者：
Kodamatani, Hitoshi;Kubo, Shotaro;Tomiyasu, Takashi
亚硝酸盐（NO2-）和硝酸盐（NO3-）离子的灵敏检测方法对于了解氮循环以及环境保护和公众健康至关重要。在此，我们报告了一种检测方法，结合离子色谱分离NO2-和NO3-，在线光化学转化这些离子的过氧亚硝酸根（ONOO-）的照射与222 nm的准分子灯，和发光之间的反应鲁米诺和ONOO-。NO2-和NO3-的检测限分别为0.01和0.03 μ M，线性范围分别为0.010-2.0和0.10-3.0 μ M，进样体积为1 μ L。所提出的海水分析方法获得的结果与参考方法（基于Griess反应的自动分析仪）的结果一致。由于鲁米诺化学发光可以测量皮摩尔浓度的ONOO-，我们的方法预计能够检测到皮摩尔浓度的NO2-和NO3-，由于ONOO-的高转化率（>60%），假设污染和背景化学发光问题可以解决。这种方法有可能成为一种创新技术，用于检测各种样品中的NO2-和NO3-。
Sensitive detection methods for nitrite (NO2-) and nitrate (NO3-) ions are essential to understand the nitrogen cycle and for environmental protection and public health. Herein, we report a detection method that combines ion-chromatographic separation of NO2- and NO3-, on-line photochemical conversion of these ions to peroxynitrite (ONOO-) by irradiation with a 222 nm excimer lamp, and chemiluminescence from the reaction between luminol and ONOO-. The detection limits for NO2- and NO3- were 0.01 and 0.03 mu M, respectively, with linear ranges of 0.010-2.0 and 0.10-3.0 mu M, respectively, at an injection volume of 1 mu L. The results obtained by the proposed method for seawater analysis corresponded with those of a reference method (AutoAnalyzer based on the Griess reaction). As luminol chemiluminescence can measure ONOO- at picomolar concentrations, our method is expected to be able to detect NO2- and NO3- at picomolar concentrations owing to the high conversion ratio to ONOO- (>60%), assuming that contamination and background chemiluminescence issues can be resolved. This method has the potential to emerge as an innovative technology for NO2- and NO3- detection in various samples.