Mechanism of spacer integration links the CRISPR/Cas system to transposition as a form of mobile DNA
Mechanism of spacer integration links the CRISPR/Cas system to transposition as a form of mobile DNA
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DOI：
10.1186/s13100-015-0039-3
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发表时间：
2015-05-12
期刊：
影响因子：
4.9
通讯作者：
Hickman, Alison B.
中科院分区：
文献类型：
--
作者：
Dyda, Fred;Hickman, Alison B.
最近已经清楚，许多细菌和古细菌物种具有适应性免疫系统。这些以多拷贝的DNA序列为代表，称为成簇的规则间隔短回文重复序列（CRISPR）。这些CRISPR重复序列是整合含有先前遇到的外源DNA序列的短间隔区的位点，并且间隔区充当先前入侵者的分子记忆。体内研究表明，两种CRISPR相关蛋白-Cas 1和Cas 2-是间隔区整合所必需的，但实现这一点的机制仍不清楚。在这里，我们回顾了最近的一篇论文，描述了使用纯化的Cas 1和Cas 2体外重建CRISPR间隔区整合，并将结果置于类似的DNA转座反应和Cas 1/Cas 2复合物的晶体结构的背景下。
It has recently become clear that many bacterial and archaeal species possess adaptive immune systems. These are typified by multiple copies of DNA sequences known as clustered regularly interspaced short palindromic repeats (CRISPRs). These CRISPR repeats are the sites at which short spacers containing sequences of previously encountered foreign DNA are integrated, and the spacers serve as the molecular memory of previous invaders. In vivo work has demonstrated that two CRISPR-associated proteins - Cas1 and Cas2 - are required for spacer integration, but the mechanism by which this is accomplished remained unclear. Here we review a recent paper describing the in vitro reconstitution of CRISPR spacer integration using purified Cas1 and Cas2 and place the results in context of similar DNA transposition reactions and the crystal structure of the Cas1/Cas2 complex.