Characterisation of spinosad resistance in the housefly Musca domestica (Diptera: Muscidae).
Characterisation of spinosad resistance in the housefly Musca domestica (Diptera: Muscidae).
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DOI：
10.1002/ps.2073
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发表时间：
2011-03
影响因子：
4.1
通讯作者：
Jing Shi;Lan Zhang;Xiwu Gao
中科院分区：
文献类型：
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作者：
Jing Shi;Lan Zhang;Xiwu Gao
背景多杀菌素是一种相对较新的、有效的和安全的农药，在过去10年中已被广泛用于害虫防治。然而，世界各地的一些昆虫对这种杀虫剂产生了不同程度的抗药性。结果经27代连续筛选，获得一株抗性品系（SpRR），抗性倍数为敏感品系（CSS）的247倍。多杀菌素抗性的估计实现遗传力（h（2））为0.14。正反交F（1）和F（1）'的LD（50）值和斜率差异不显著，显性度分别为0.33（F（1））和0.30（F（1）'）。对自交（F（2））和回交（BC（1）和BC（2））的卡方分析均达到极显著水平，表明该抗性可能受多个基因控制。增效剂胡椒基丁醚（PBO）、马来酸二乙酯（DEM）和S，S，S-三丁基三硫代磷酸酯（DEF）对多杀菌素的毒力影响较小，说明代谢介导的解毒作用不是SpRR菌株产生多杀菌素抗性的重要因素。结论家蝇对多杀菌素的抗性为常染色体不完全显性遗传，可能受多基因控制。这些结果为设计成功的家蝇防治方案提供了基本信息。
BACKGROUND Spinosad, a relatively new, effective and safe pesticide, has been widely used in pest control over the last 10 years. However, different levels of resistance to this insecticide have developed in some insects worldwide. RESULTS After continuous selection for 27 generations, a strain (SpRR) of the housefly developed 247-fold resistance to spinosad compared with the laboratory susceptible strain (CSS). The estimated realised heritability (h(2) ) of spinosad resistance was 0.14. There was no significant difference in the LD(50) values and slopes between reciprocal progenies F(1) and F(1) ', and values of 0.33 (F(1) ) and 0.30 (F(1) ') were obtained for the degree of dominance. Chi-square analysis from responses of self-bred (F(2) ) and backcrosses (BC(1) and BC(2) ) were highly significant, suggesting that the resistance was probably controlled by more than one gene. Synergists piperonyl butoxide (PBO), diethyl maleate (DEM) and S,S,S-tributyl phosphorotrithioate (DEF) affected the toxicity of spinosad at a low level, and demonstrated that metabolic-mediated detoxification was not an important factor in conferring resistance to spinosad in the SpRR strain. CONCLUSION It was concluded that spinosad resistance in the housefly was autosomal and incompletely dominant, and the resistance was probably controlled by more than one gene. These results provide the basic information for designing successful management programmes for the control of houseflies.