Molecular and biological properties of a macrophage colony-stimulating factor from mouse yolk sacs.
Molecular and biological properties of a macrophage colony-stimulating factor from mouse yolk sacs.
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DOI：
10.1083/jcb.77.1.35
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发表时间：
1978-04
期刊：
影响因子：
--
通讯作者：
Burgess AW
中科院分区：
文献类型：
--
作者：
Johnson GR;Burgess AW
从小鼠卵黄囊条件培养液（YSCM）中部分纯化并浓缩了集落刺激因子（M-CSF）。M-CSF似乎优先刺激CBA骨髓粒细胞-巨噬细胞祖细胞（GM-CFC）在半固体琼脂培养物中分化形成巨噬细胞集落。通过比较，来自小鼠肺条件培养基（MLCM）的集落刺激因子（GM-CSF）刺激粒细胞、混合粒细胞-巨噬细胞和纯巨噬细胞集落的形成。混合实验表明，M-CSF和GM-CSF均能刺激所有的GM-CFC，但较小的CFC对GM-CSF更敏感，较大的CFC对M-CSF更敏感。几乎所有最初用M-CSF刺激的发育“克隆”在转移到含有GM-CSF的培养物中时继续发育。在匡威的情况下，当转移到含有M-CSF的培养物中时，只有50%的GM-CSF预刺激的“克隆”存活。最初由M-CSF刺激或转移到由M-CSF刺激的培养物中的所有克隆在培养7天后含有巨噬细胞。这些结果表明，存在能够分化形成粒细胞和巨噬细胞的细胞群（GM-CFC），但是，一旦这些细胞被特定的CSF（例如M-CSF）激活，它们就被定向于特定的分化途径。CFC分化的模式与增殖速率没有直接关系：由M-CSF最大程度刺激的培养物产生大多数巨噬细胞集落，但少量GM-CSF的存在下产生30%的集落中的粒细胞。凝胶过滤、十二烷基硫酸钠存在下的聚丙烯酰胺凝胶电泳和伴刀豆球蛋白A-Sepharose亲和层析表明，来自卵黄囊的M-CSF是表观分子量为60，000的糖蛋白。伴刀豆球蛋白A-Sepharose层析证明，该分子的碳水化合物部分存在一定的异质性。
A colony-stimulating factor (M-CSF) has been partially purified and concentrated from mouse yolk sac-conditioned medium (YSCM). M-CSF appeared to preferentially stimulate CBA bone marrow granulocyte- macrophage progenitor cells (GM-CFC) to differentiate to form macrophage colonies in semisolid agar cultures. By comparison, colony- stimulating factor (GM-CSF) from mouse lung-conditioned medium (MLCM) stimulated the formation of granulocytic, mixed granulocytic- macrophage, and pure macrophage colonies. Mixing experiments indicated that both M-CSF and GM-CSF stimulated all of the GM-CFC but that the smaller CFC were more sensitive to GM-CSF and that the larger CFC were more sensitive to M-CSF. Almost all developing "clones" stimulated initially with M-CSF continued to develop when transferred to cultures containing GM-CSF. In the converse situation, only 50% of GM-CSF prestimulated "clones" survived when transferred to cultures containing M-CSF. All clones initially stimulated by M-CSF or transferred to cultures stimulated by M-CSF contained macrophages after 7 days of culture. These results suggest that there is a population of cells (GM- CFC) that are capable of differentiating to form both granulocytes and macrophages, but, once these cells are activated by a specific CSF (e.g. M-CSF), they are committed to a particular differentiation pathway. The pattern of CFC differentiation was not directly related to the rate of proliferation: cultures maximally stimulated by M-CSF produced mostly macrophage colonies, but the presence of small amounts of GM-CSF produced granulocytic cells in 30% of the colonies. Gel filtration, polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, and affinity chromatography with concanavalin A- Sepharose indicated that M-CSF from yolk sacs was a glycoprotein with an apparent molecular weight of 60,000. There was some heterogeneity of the carbohydrate portion of the molecule as evidenced by chromatography on concanavalin A-Sepharose.