Calcium spikes accompany cleavage furrow ingression and cell separation during fission yeast cytokinesis.
Calcium spikes accompany cleavage furrow ingression and cell separation during fission yeast cytokinesis.
复制标题
DOI：
10.1091/mbc.e20-09-0609
复制
发表时间：
2021-01-01
影响因子：
3.3
通讯作者：
Chen Q
中科院分区：
文献类型：
--
作者：
Poddar A;Sidibe O;Ray A;Chen Q
钙信号在细胞分裂中的作用一直不明确。过去对胚胎细胞分裂的研究发现，在分裂平面上，就在卵裂沟进入之前，钙浓度会短暂增加，这表明这些钙瞬态可能会引发收缩环收缩。然而，这种钙瞬变体只在动物胚胎中发现，其功能仍有争议。我们采用基因编码的钙指示剂GCaMP测定裂糖酵母（Schizosaccharomyces pombe）胞内钙水平，探讨裂糖酵母（Schizosaccharomyces pombe）细胞动力学钙瞬态。我们验证了GCaMP是裂变酵母中高度敏感的钙报告蛋白，使我们能够捕获渗透冲击触发的钙瞬态。我们发现细胞内钙水平与细胞分裂之间存在相关性，这与细胞分裂过程中钙瞬态的存在一致。利用延时显微镜和定量图像分析，我们发现在卵裂沟进入开始和细胞分离结束时都有钙峰。抑制这些钙峰值减缓了沟的侵入，导致子细胞频繁裂解。我们得出结论，与较大的动物胚胎一样，裂变酵母触发钙瞬变，这可能在细胞质分裂中起重要作用（197）。
The role of calcium signaling in cytokinesis has long remained ambiguous. Past studies of embryonic cell division discovered that calcium concentration increases transiently at the division plane just before cleavage furrow ingression, suggesting that these calcium transients could trigger contractile ring constriction. However, such calcium transients have only been found in animal embryos and their function remains controversial. We explored cytokinetic calcium transients in the fission yeast Schizosaccharomyces pombe by adopting GCaMP, a genetically encoded calcium indicator, to determine the intracellular calcium level of this model organism. We validated GCaMP as a highly sensitive calcium reporter in fission yeast, allowing us to capture calcium transients triggered by osmotic shocks. We identified a correlation between the intracellular calcium level and cell division, consistent with the existence of calcium transients during cytokinesis. Using time-lapse microscopy and quantitative image analysis, we discovered calcium spikes both at the start of cleavage furrow ingression and the end of cell separation. Inhibition of these calcium spikes slowed the furrow ingression and led to frequent lysis of daughter cells. We conclude that like the larger animal embryos, fission yeast triggers calcium transients that may play an important role in cytokinesis (197).