H-NS Silences gfp, the Green Fluorescent Protein Gene: gfpTCD Is a Genetically Remastered gfp Gene with Reduced Susceptibility to H-NS-Mediated Transcription Silencing and with Enhanced Translation
H-NS Silences gfp, the Green Fluorescent Protein Gene: gfpTCD Is a Genetically Remastered gfp Gene with Reduced Susceptibility to H-NS-Mediated Transcription Silencing and with Enhanced Translation
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DOI：
10.1128/jb.00531-10
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发表时间：
2010-09-01
影响因子：
3.2
通讯作者：
Dorman, Charles J.
中科院分区：
文献类型：
--
作者：
Corcoran, Colin P.;Cameron, Andrew D. S.;Dorman, Charles J.
细菌核相关蛋白H-NS优先靶向和沉默富含A+ t的基因，与无处不在的报告基因gfp结合，显著减少局部转录。我们重新设计了gfp，以减少h - ns介导的转录沉默，同时在不改变gfp蛋白氨基酸序列的情况下改善体内翻译。
The bacterial nucleoid-associated protein H-NS, which preferentially targets and silences A+T-rich genes, binds the ubiquitous reporter gene gfp and dramatically reduces local transcription. We have redesigned gfp to reduce H-NS-mediated transcription silencing and simultaneously improve translation in vivo without altering the amino acid sequence of the GFP protein.