Expression and localization of CXCL16 and CXCR6 in ovarian endometriotic tissues
Expression and localization of CXCL16 and CXCR6 in ovarian endometriotic tissues
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DOI：
10.1007/s00404-011-2002-y
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发表时间：
2011-07
影响因子：
2.6
通讯作者：
S. Manabe;A. Iwase;Maki Goto;H. Kobayashi;S. Takikawa;Yoshinari Nagatomo;T. Nakahara;Bayasula
中科院分区：
文献类型：
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作者：
S. Manabe;A. Iwase;Maki Goto;H. Kobayashi;S. Takikawa;Yoshinari Nagatomo;T. Nakahara;Bayasula
目的：炎症介质，包括趋化因子，可能在子宫内膜异位症的发生发展中起重要作用。因此，我们研究了CXCL16及其受体CXCR6在卵巢增生组织中的表达和定位。我们还研究了CXCL16是否诱导IL-8的产生在adenotic stromal cells. MethodsWe进行免疫组化和Western blotting分析的体内和体外样品。结果CXCL 16和CXCR 6均在卵巢上皮细胞和间质细胞中表达，而在正常卵巢间质中不表达。使用原代培养的前列腺间质细胞的Western印迹分析显示，在增殖期、分泌期和促性腺激素释放激素激动剂治疗期间，CXCL 16和CXCR 6的表达恒定。结论CXCL16和CXCR6可能通过调节炎症反应参与子宫内膜异位症的病理生理过程。
PurposeInflammatory mediators, including chemokines, may play crucial roles in the development of endometriosis. Therefore, we investigated the expression and localization of CXCL16 and its receptor, CXCR6, in ovarian endometriotic tissues. We also examined whether CXCL16 induces IL-8 production in endometriotic stromal cells.MethodsWe performed immunohistochemical and Western blotting analyses of in vivo and in vitro samples. IL-8 production was assayed using an ELISA.ResultsBoth CXCL16 and CXCR6 were expressed by endometriotic epithelial cells and stromal cells, but not normal ovarian stroma. A Western blotting analysis using primary cultured endometriotic stromal cells showed a constant expression of CXCL16 and CXCR6 in the proliferative phase, secretory phase and during gonadotropin-releasing hormone agonist therapy. CXCL16 induced IL-8 production in several endometriotic stromal cells in vitro.ConclusionsCXCL16 and CXCR6 might be involved in the pathophysiology of endometriosis through regulation of the inflammatory response.