Extraction of antibacterial compounds from Combretum microphyllum (Combretaceae)
Extraction of antibacterial compounds from Combretum microphyllum (Combretaceae)
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DOI：
10.1016/s0254-6299(15)30442-7
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发表时间：
2002-03-01
影响因子：
3.1
通讯作者：
Eloff, JN
中科院分区：
文献类型：
--
作者：
Kotzé, M;Eloff, JN
本研究的目的是通过使用不同的萃取剂来简化萃取物，以便从植物中复杂的化学物质混合物中分离出抗菌化合物。用丙酮和1%碳酸氢钠水溶液提取完整的干叶，用己烷、四氯化碳、二异丙醚、乙醚、二氯二甲基甲烷、四氢呋喃、丙酮、乙醇、乙酸乙酯、甲醇和水提取碎叶。采用薄层色谱法测定提取物的化学成分，采用微孔板串联稀释法测定提取物的抑菌活性。不同溶剂提取率为干重的2.6 ~ 17.4%。甲醇、二氯二甲基和四氢呋喃萃取成分最多。不同提取物的非极性成分的化学成分非常相似。不同萃取剂对金黄色葡萄球菌、铜绿假单胞菌、大肠杆菌和粪肠球菌的最低抑菌浓度为0.01 ~ 1.25 mg/ml。提取物对革兰氏阴性菌和革兰氏阳性菌具有相似的活性。二异丙醚、乙醇、乙醚、丙酮和乙酸乙酯对其他非活性化合物的抑菌活性较高，可用于分离生物活性化合物。
The aim of this investigation was to simplify extracts to facilitate the isolation of antibacterial compounds from the complex mixture of chemicals in the plant by using different extractants. Intact dried leaves were extracted with acetone and 1% aqueous sodium bicarbonate and ground leaves were extracted by hexane, carbon tetrachloride, di-isopropylether, ethyl ether, methylene dichloride, tetrahydrofuran, acetone, ethanol, ethyl acetate, methanol and water. TLC was used to determine chemical composition and antibacterial activity of extracts was determined by a microplate serial dilution method. The different solvents extracted from 2.6 to 17.4% of the dry weight. Methanol, methylene dichloride and tetrahydrofuran extracted the most components. The chemical composition of the non-polar components of the different extracts were remarkably similar. The minimum inhibitory concentration for the different extractants varied from 0.01 to 1.25 mg/ml with the four test organisms used (Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Enterococcus faecalis). The extracts had similar activity towards Gram-negative and Gram-positive bacteria. Di-isopropyl ether, ethanol, ethyl ether, acetone and ethyl acetate extracted high antibacterial activity with a lower quantity of other non-active compounds and could be useful for isolating bioactive compounds.