Quantitative comparison of the passage of homologous and heterologous spermatozoa through the uterotubal junction of the golden hamster.
Quantitative comparison of the passage of homologous and heterologous spermatozoa through the uterotubal junction of the golden hamster.
复制标题
同源和异源精子通过金黄地鼠子宫输卵管连接处的定量比较。
DOI：
10.1002/mrd.1120190302
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Yanagimachi,R
中科院分区：
文献类型：
--
作者：
Smith,TT;Koyanagi,F;Yanagimachi,R
采用定量方法确定外来种属精子是否能像同源（仓鼠）精子一样有效地通过仓鼠子宫输卵管连接部（UTJ）。用同源和异源（外来）物种的附睾精子对动情的雌性仓鼠进行人工授精。授精后数小时（排卵前不久）测定输卵管中精子的数量和分布。还检查了不活动（死亡）仓鼠精子通过UTJ的情况，发现所有供试外来种属（大鼠、小鼠、豚鼠和兔）的精子以及不活动仓鼠精子都可以通过UTJ，但与活仓鼠精子相比，其数量要少得多。这并不是由于仓鼠子宫中外来精子存活率差所致，因为所有授精精子（包括仓鼠精子）的活力在授精后1 h显著降低。虽然在检查时大量活仓鼠精子分布在整个尾峡部，但没有或只有极少数外来精子前进到此为止。到达尾峡部的少数外来和不动精子仅限于该节段的第一个上升环。本文还讨论了仓鼠输卵管中外来精子数量少、推进迟缓的可能原因。
A quantitative method was used to determine whether the spermatozoa of foreign species could pass through the uterotubal junction (UTJ) of the hamster as efficiently as homologous (hamster) spermatozoa. Estrous female hamsters were artificially inseminated with epididymal spermatozoa of homologous and heterologous (foreign) species. The number and distribution of spermatozoa in the oviduct were determined several hours after insemination (shortly before ovulation). The passage of immotile (dead) hamster spermatozoa through the UTJ was also examined.It was found that the spermatozoa of all foreign species tested (rat, mouse, guinea pig, and rabbit), as well as immotile hamster spermatozoa, could pass through the UTJ but did so in much smaller numbers compared to live hamster spermatozoa. This was not specifically due to poor survival of foreign spermatozoa in the hamster uterus, as the viability of all inseminated spermatozoa (including hamster spermatozoa) was considerably reduced by 1 h after insemination. While a large number of live hamster spermatozoa were distributed throughout the caudal isthmus at the time of examination, none or only a very few foreign spermatozoa had advanced this far. The few foreign and immotile spermatozoa that reached the caudal isthmus were confined to the first ascending loop of this segment. Some possible causes for the small number and retarded advance of foreign spermatozoa in the hamster oviduct were discussed.