Heme oxygenase-carbon monoxide (HO-CO) system in rat uterus: Effect of sexual steroids and prostaglandins
Heme oxygenase-carbon monoxide (HO-CO) system in rat uterus: Effect of sexual steroids and prostaglandins
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DOI：
10.1016/j.jsbmb.2005.11.007
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发表时间：
2006-04-01
影响因子：
4.1
通讯作者：
Franchi, Ana M.
中科院分区：
文献类型：
--
作者：
Cella, Maximiliano;Farina, Mariana G.;Franchi, Ana M.
妊娠维持是一个非常复杂的现象，并且允许胎儿在母体子宫内存活的机制仍然知之甚少。我们的目的是分析血红素氧合酶（HO）的活性和表达在怀孕的大鼠，并研究其与类固醇激素和三尖杉酯碱的关联。在妊娠第5、13、18-22天和产后，从非妊娠大鼠和同期交配大鼠中获得子宫组织。HO活性在第5和20天显著升高，而HO-1蛋白水平在第19 ~ 22天显著升高。在去卵巢大鼠中，雌激素和孕激素使HO活性和表达增加。环氧合酶抑制剂增加HO活性和HO-1表达。用前列腺素F-2 α（PGF（2 α））预孵育可降低去卵巢大鼠子宫中的酶活性。锡原卟啉IX，HO抑制剂，显着降低子宫cGMP的积累。胆红素降低子宫硫代巴比妥酸物质水平（脂质过氧化的指标）。这些结果表明，子宫妊娠模式的HO活性和表达的大鼠。此外，这些结果表明，子宫HO活性可能通过cGMP调节妊娠子宫静止，并可能有助于防御氧化应激。(c)2006爱思唯尔有限公司保留所有权利。
Pregnancy maintenance is a very complex phenomenon, and the mechanisms that allow the survival of the fetus within the maternal uterus are still poorly understood. Our objectives were to analyze heme oxygenase (HO) activity and expression in the pregnant rat and to study its association with steroid hormones and prostaglandins. Uterine tissues were obtained from non-pregnant and from time-mated rats at days 5, 13, 18-22 of pregnancy and postpartum. HO activity was significantly higher at days 5 and 20 while HO-1 protein levels measured by Western blot, were significantly elevated from days 19 to 22.In ovariectomized rats, estrogen and progesterone in estrogenized animals increased HO activity and expression. Cyclooxygenase inhibitors augmented HO activity and HO-1 expression. Pre-incubation with prostaglandin F-2 alpha (PGF(2 alpha)) diminished the enzymatic activity in ovariectomized rat uterus. Tin protoporphyrin IX, an HO inhibitor, significantly decreased uterine cGMP accumulation. Bilirubin decreased uterine thiobarbituric acid substances levels (an index of lipid peroxidation).These results demonstrate a uterine gestational pattern of HO activity and expression in the rat. In addition, these results suggest that uterine HO activity could regulate uterine quiescence in pregnancy via cGMP and it may contribute to the defense against oxidative stress. (c) 2006 Elsevier Ltd. All rights reserved.