Revisiting with a Relative-Density Calibration Approach the Determination of Growth Rates of Microorganisms by Use of Optical Density Data from Liquid Cultures
Revisiting with a Relative-Density Calibration Approach the Determination of Growth Rates of Microorganisms by Use of Optical Density Data from Liquid Cultures
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DOI：
10.1128/aem.00824-09
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发表时间：
2010-03-01
影响因子：
4.4
通讯作者：
Kuo, Tai-Chih
中科院分区：
文献类型：
--
作者：
Lin, Hsiu-Li;Lin, Chien-Chung;Kuo, Tai-Chih
为了解决从光密度(OD)-生长时间图测量微生物生长速度的问题，我们使用了相对密度(RD)图。用稀释后的培养物建立OD与RD的关系。该方法用于大肠杆菌和螺旋藻的生长研究，结果令人满意。
To solve the problems of measuring the growth rates of microorganisms from optical density (OD)-growth time plots, we used relative-density (RD) plots. The relationship of OD and RD was built from the diluted grown cultures. This method was satisfactorily applied to study the growth of Escherichia coli and the cyanobacterium Anabaena spiroides.