Halalkalibacillus sediminis sp. nov., isolated from sediment of sea cucumber culture pond
Halalkalibacillus sediminis sp. nov., isolated from sediment of sea cucumber culture pond
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Halalkalibacillus sediminis sp.
DOI：
10.1099/ijsem.0.003927
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发表时间：
2020
影响因子：
2.8
通讯作者：
Zong-Jun Du
中科院分区：
文献类型：
--
作者：
Rui-Han He;Zhen-Wei Liu;Yu-Qi Yan;Zong-Jun Du
从中国荣成（122.2° E36.9 ° N）海参养殖池的沉积物中分离到一株革兰氏染色阳性、杆状（0.3-0.4×1.2-2.0 µm）、严格好氧、米色的细菌，命名为B3227 T。其生化特性分析表明，该菌细胞为过氧化氢酶阳性，氧化酶阴性。细胞生长发生在15-45 °C（最佳，37 °C）、pH 6.5-9.0（pH 7.5-8.0）和0.0- 22.0%（w/v）NaCl（6.0- 9.0%NaCl）存在下。 16 S rRNA基因序列分析表明，菌株B3227 T与Milensis、Piscibacillussalipiscarius、Halalkalibacillushalophilus和Piscibacillushalophilus模式菌株的相似性分别为95.7%、95.5%、95.5%和95.3%，生理生化分析表明菌株B3227 T与Halalkalibacillusgenus的相似性最高。 这些细胞是内孢子形成的，并且包含A1-γ-内消旋-二氨基庚二酸型肽聚糖。菌株B3227 T的呼吸醌为MK-7，主要脂肪酸为antiso-C15：0和antiso-C17：0。 极性脂质主要为二磷脂酰甘油和磷脂酰乙醇胺。基因组DNA G+C含量为38.7mol%。菌株B3227与H之间的平均核苷酸同一性值。嗜盐菌JCM 14192 T（ANIb 69.5%，ANIm 84.2%）和F. milensisJCM 12288 T（ANIb 70.1%，ANIm 84.1%）低于物种划分的截止水平（95- 96%）。 酮分析的结果显示，菌株B3227 T可以生物合成莽草酸，一种用于配制猪流感药物的基本化合物。根据菌株B3227 T的形态特征、生理特性以及系统发育的独特性，菌株B3227 T应归属于Halalkalibacillus属，为Halalkalibacillussediminissp.十一是建议。模式菌株为B3227 T（=KCTC 33093 T =MCCC 1H 00193 T）。
A Gram-stain-positive, rod-shaped (0.3–0.4×1.2–2.0 µm), strictly aerobic and beige-pigmented bacterium, designated B3227T, was isolated from the sediment of a sea cucumber culture pond in Rongcheng, China (122.2° E 36.9° N). Its biochemical characteristics analysis revealed that the cells of this bacterium were catalase-positive and oxidase-negative. Cell growth occurred at 15–45 °C (optimum, 37 °C), pH 6.5–9.0 (pH 7.5–8.0) and in the presence of 0.0–22.0 % (w/v) NaCl (6.0–9.0 % NaCl). Phylogenetic analysis based on 16S rRNA gene sequencing indicated that strain B3227Texhibited similarities of 95.7, 95.5, 95.5 and 95.3 % to the type strains ofFilobacillus milensis,Piscibacillus salipiscarius,Halalkalibacillus halophilusandPiscibacillus halophilus, respectively, and the results of physiological analyses revealed that strain B3227Twas most similar to the genusHalalkalibacillus. The cells were endospore-forming and comprised an A1-γ-meso-diaminopimelic acid-type peptidoglycan. The respiratory quinone of strain B3227Twas MK-7, and the dominant fatty acids were anteiso-C15 : 0and anteiso-C17 : 0. The major polar lipids were diphosphatidylglycerol and phosphatidylethanolamine. The genomic DNA G+C content was 38.7 mol%. The average nucleotide identity values between strain B3227TandH. halophilusJCM 14192T(ANIb 69.5%, ANIm 84.2 %) andF. milensisJCM 12288T(ANIb 70.1 %, ANIm 84.1 %) were below the cut-off level (95–96 %) for species delineation. The results ofkegganalysis revealed that strain B3227Tcould biosynthesize shikimate acid, a base compound for the formulation of the swine flu drug. Based on its morphological and physiological properties, as well as phylogenetic distinctiveness, strain B3227Tshould be placed into the genusHalalkalibacillusas a representative of a new species, for which the nameHalalkalibacillus sediminissp. nov. is proposed. The type strain is B3227T(=KCTC 33093T=MCCC 1H00193T).