Biological characteristics and salt-tolerant plant growth-promoting effects of an ACC deaminase-producing Burkholderia pyrrocinia strain isolated from the tea rhizosphere
Biological characteristics and salt-tolerant plant growth-promoting effects of an ACC deaminase-producing Burkholderia pyrrocinia strain isolated from the tea rhizosphere
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茶叶根际产ACC脱氨酶伯克霍尔德氏菌的生物学特性及耐盐植物促生作用
DOI：
10.1007/s00203-021-02204-x
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发表时间：
2021-03-01
影响因子：
2.8
通讯作者：
Zhou, Jing
中科院分区：
文献类型：
--
作者：
Han, Lizhen;Zhang, Hong;Zhou, Jing
产生1-氨基环丙烷-1-羧酸（ACC）脱氨酶的植物根际促生细菌可以促进植物生长并增强非生物胁迫耐受性。在这项研究中，从茶叶根际分离出了具有ACC脱氨酶活性为33.01-µmol/h/mg蛋白的吡咯伯克霍尔德菌菌株P10，并根据形态学，生化和分子特征进行了鉴定。除了在pH5.0 -9.0的ACC脱氨酶活性和对5%NaCl和20%聚乙二醇的响应外，菌株P10还可以溶解磷化合物，产生吲哚-3-乙酸，并分泌铁载体。盆栽试验结果表明，P10菌株在100和170 mmol/L NaCl条件下能显著促进花生幼苗的生长。与高盐胁迫相比，其鲜重和根长分别增加了90.12%和79.22%。这些结果提供了新的见解伯克霍尔德氏菌pyrrocinia，这可能是有用的生物肥料的生物学特性。
Plant growth-promoting rhizobacteria that produce 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase can promote plant growth and enhance abiotic stress tolerance. In this study, Burkholderia pyrrocinia strain P10, with an ACC deaminase activity of 33.01-µmol/h/mg protein, was isolated from the tea rhizosphere and identified based on morphological, biochemical, and molecular characteristics. In addition to its ACC deaminase activity at pH 5.0-9.0 and in response to 5% NaCl and 20% polyethylene glycol, strain P10 can also solubilize phosphorus compounds, produce indole-3-acetic acid, and secrete siderophores. Pot experiments revealed that strain P10 can significantly enhance peanut seedling growth under saline conditions (100- and 170-mmol/L NaCl). Specifically, it increased the fresh weight and root length of plants by 90.12% and 79.22%, respectively, compared with high-salt stress. These results provide new insights into the biological characteristics of Burkholderia pyrrocinia, which may be useful as a bio-fertilizer.