Genomic organization of rat prolactin and growth hormone genes.
Genomic organization of rat prolactin and growth hormone genes.
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大鼠催乳素和生长激素基因的基因组组织。
DOI：
10.1073/pnas.77.8.4583
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发表时间：
1980
影响因子：
11.1
通讯作者：
E. Thompson
中科院分区：
文献类型：
--
作者：
Y. Chien;E. Thompson
从染色体DNA文库中分离出5个含有大鼠催乳素基因及其侧翼序列的重叠克隆DNA，以及1个含有大鼠生长激素基因及其侧翼序列的克隆DNA。用限制性内切酶作图和电子显微镜对它们进行了表征。在每个基因中，编码长度约为1千碱基的成熟mRNA的结构基因序列被至少四个中间序列分裂成至少五个片段。这两个基因在编码区域的长度和组织上相似，这与它们来自共同祖先基因的说法一致。然而，这两个基因在其介入序列的长度上有很大的不同。这导致催乳素基因的总长度为10千碱基对，生长激素基因的总长度为2.1千碱基对。至少有一个中间序列出现在催乳素和生长激素mRNA编码序列的5'非翻译区。
Five overlapping cloned DNAs containing the rat prolactin gene and its flanking sequences, as well as one cloned DNA containing the rat growth hormone gene and its flanking sequences, were isolated from a chromosomal DNA library. They were characterized by restriction enzyme mapping and electron microscopy. In each gene, the structural gene sequence coding for mature mRNA of a length of about 1 kilobase is split into at least five segments by a minimum of four intervening sequences. The two genes are similar in the length and organization of their coding regions, consistent with the suggestion that they are derived from a common ancestral gene. However, the two genes differ greatly in the lengths of their intervening sequences. That leads to a total gene length of 10 kilobase pairs for the prolactin and 2.1 kilobase pairs for the growth hormone gene. At least one intervening sequence appears to be in the 5' nontranslated regions of the prolactin and growth hormone mRNA coding sequences.