Comparison of three nonviral transfection methods for foreign gene expression in early chicken embryos in ovo
Comparison of three nonviral transfection methods for foreign gene expression in early chicken embryos in ovo
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DOI：
10.1006/bbrc.1996.5882
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发表时间：
1997-01-13
影响因子：
3.1
通讯作者：
Okumura, J
中科院分区：
文献类型：
--
作者：
Muramatsu, T;Mizutani, Y;Okumura, J
通过使用三种非病毒转染方法，比较了微粒子轰击法、脂质体转染法和电穿孔法在鸡卵内发育胚胎中的转染效率和lacZ报告基因的表达强度。在所采用的三种转染方法中，电穿孔赋予细菌lacZ基因最强的表达，具有相似的转染效率。结果表明，就瞬时基因表达而言，电穿孔将提供一种有用和有效的外源基因转染到活鸡胚体细胞的非病毒手段。(C)1997学术出版社
By using three nonviral transfection methods, i.e., microparticle bombardment, lipofection and electroporation, the transfection efficiency and the expression intensity of a lacZ reporter gene were compared in developing chicken embryos in ovo. Of the three transfection methods employed, electroporation conferred the strongest expression of the bacterial lacZ gene with similar transfection efficiency. The results suggest that as far as transient gene expression is concerned, electroporation would provide a useful and efficient nonviral means of foreign gene transfection to somatic cells of living chicken embryos in ovo. (C) 1997 Academic Press