Effects of parathyroid hormone on cytosolic calcium in renal proximal tubular primary cultures.
Effects of parathyroid hormone on cytosolic calcium in renal proximal tubular primary cultures.
复制标题
甲状旁腺激素对肾近端肾小管原代培养物中胞质钙的影响。
DOI：
10.1152/ajprenal.1986.251.2.f188
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Moskowitz,D
中科院分区：
文献类型：
--
作者：
Hruska,KA;Goligorsky,M;Scoble,J;Tsutsumi,M;Westbrook,S;Moskowitz,D
用荧光钙离子指示剂quin 2测定了甲状旁腺激素对原代培养的犬肾近曲小管细胞胞浆钙离子浓度的影响。培养的细胞表现出对激素、酶活性、运输功能和近曲小管形态特征的反应。甲状旁腺激素刺激细胞质Ca 2+的立即上升，无论是在悬浮细胞和细胞作为一个单层的Nuclepore过滤器上研究。由激素诱导的细胞质Ca 2+的升高持续15-30分钟，呈剂量依赖性，并且不被环AMP模仿。从细胞外液中去除Ca ~（2+）可显著降低胞浆Ca ~（2+），并消除甲状旁腺激素对胞浆Ca ~（2+）的影响。8-（N，N-二乙基氨基）辛基-3，4，5-三甲氧基苯甲酸酯阻断激素对胞浆Ca 2+的作用，但线粒体解偶联剂未能抑制激素增加胞浆Ca 2+的作用。这些研究支持的作用，Ca 2+的激活近端肾小管细胞的甲状旁腺激素。
The effects of parathyroid hormone on the cytoplasmic Ca2+ concentration of canine renal proximal tubule cells grown in primary culture were determined using the fluorescent Ca2+ indicator quin 2. The cultured cells exhibited responses to hormones, enzyme activities, transport functions, and morphology characteristic of the proximal convoluted tubule. Parathyroid hormone stimulated an immediate rise in cytoplasmic Ca2+, both in suspended cells and cells studied as a monolayer on Nuclepore filters. The rise in cytoplasmic Ca2+ induced by the hormone was sustained for 15-30 min, was dose dependent, and was not mimicked by cyclic AMP. Removing Ca2+ from the extracellular media markedly decreased cytoplasmic Ca2+ and abolished the effects of parathyroid hormone on cytosolic Ca2+. 8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate blocked the effects of the hormone on cytosolic Ca2+, but mitochondrial uncouplers failed to inhibit the effects of the hormone to increase cytoplasmic Ca2+. These studies support a role of Ca2+ in the activation of proximal renal tubule cells by parathyroid hormone.