Kinamycin acetyltransferase I from Streptomyces murayamaensis, an apparently large, membrane-associated enzyme.
Kinamycin acetyltransferase I from Streptomyces murayamaensis, an apparently large, membrane-associated enzyme.
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来自村山链霉菌的动霉素乙酰转移酶 I，一种明显较大的膜相关酶。
DOI：
10.1016/0968-0896(96)00097-1
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发表时间：
1996
影响因子：
3.5
通讯作者：
Tang,Z
中科院分区：
文献类型：
--
作者：
Gould,SJ;O'Hare,T;Seaton,P;Soodsma,J;Tang,Z
描述了一种明显较大的膜相关多功能酶--激动素乙酰转移酶I(KAT-1)的鉴定和初步性质。Kat I活性比无细胞提取液高29倍。在部分纯化过程中，两种乙酰基转移酶催化依赖于乙酰辅酶A的激动素F和E分别转化为激动素E和D是密不可分的。部分纯化包括差速超速离心法从胞浆蛋白中分离Kat I，用0.5%CHAPS两性离子洗涤剂增溶，然后超速离心法，以及Sephacryl S400凝胶过滤层析上清液。
Identification and initial characterization of an apparently large, membrane-associated multifunctional enzyme, kinamycin acetyltransferase I (KAT 1), is described. KAT I activity was enriched 29-fold over the level in cell-free extracts of Streptomyces murayamaensis. Two acetyltransferase activities catalyzing acetyl coenzyme A dependent conversion of kinamycin F and E to kinamycin E and D, respectively, were inseparable in the course of the partial purification. Partial purification involved separation of KAT I from cytosolic proteins by differential ultracentrifugation, solubilization with 0.5% CHAPS zwitterionic detergent followed by ultracentrifugation, and Sephacryl S400 gel filtration chromatography of the resulting supernatant.