Sodium iodoacetate-induced experimental osteoarthritis and associated pain model in rats
Sodium iodoacetate-induced experimental osteoarthritis and associated pain model in rats
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DOI：
10.1292/jvms.65.1195
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发表时间：
2003-11-01
影响因子：
1.2
通讯作者：
Komatsu, H
中科院分区：
文献类型：
--
作者：
Kobayashi, K;Imaizumi, R;Komatsu, H
通过关节内注射软骨细胞代谢抑制剂单碘乙酸盐（MIA），以0、0.3或3 mg/关节的剂量在Wistar大鼠膝关节中诱导退行性病变。进行组织学检查和用失能测试仪测量作为关节疼痛指标的后爪重量比。与人类骨关节炎（OA）相似的组织学发现，如软骨细胞解体、软骨表面侵蚀和纤维化以及软骨下骨暴露等，以MIA剂量依赖性方式观察。番红花素-O快绿色染色显示，MIA处理的大鼠软骨组织中蛋白聚糖明显弥漫性减少。关节疼痛的临床评分与组织学分级密切相关。我们的结论是，目前的实验模型结合一种新的双通道重量平均器将是非常有用的人类OA的研究，并可用于估计新的抗OA药物的治疗效果。
Degenerative lesions were induced in the knee joint of Wistar rats by intraarticular injection of chondrocyte metabolism inhibitor mono-iodoacetate (MIA) at doses of 0, 0.3 or 3 mg/joint. Histopathological examination and the measurement of hind paw weight ratio as an index of joint pain by incapacitance tester were performed. Histological findings that are similar to those observed in human osteoarthritis (OA), such as disorganization of chondrocytes, erosion and fibrillation of cartilage surface, and subchondral bone exposure etc., were observed in a MIA-dose-dependent manner. Saflanin-O fast green staining revealed that marked diffuse reduction of proteoglycan in cartilage tissue of rats treated with MIA. The clinical scores of the joint pain were closely correlated to the grade of histological findings. We conclude that the present experimental model in combination with a novel dual channel weight averager would be very useful for the study of human OA, and could be applied for estimation of therapeutic effect of new anti-OA drugs.