Effect of antioxidant/reducing agents on the initial and long-term bonding performance of a self-etch adhesive to caries-affected dentin with and without smear layer-deproteinizing
Effect of antioxidant/reducing agents on the initial and long-term bonding performance of a self-etch adhesive to caries-affected dentin with and without smear layer-deproteinizing
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DOI：
10.1016/j.ijadhadh.2020.102648
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发表时间：
2020-10-01
影响因子：
3.4
通讯作者：
Nakajima, Masatoshi
中科院分区：
文献类型：
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作者：
Prasansuttiporn, Taweesak;Thanatvarakorn, Ornnicha;Nakajima, Masatoshi
本研究旨在评价含有对甲苯亚磺酸钠盐（Accel（R））和植物提取物抗氧化剂（迷迭香酸溶液）的还原剂对自酸蚀粘合剂与龋损牙本质（有和没有用NaOCl溶液去除玷污层蛋白质）的粘结耐久性的影响。实验分为两组，一组用NaOCl溶液去除牙本质玷污层蛋白，另一组用对甲苯亚磺酸钠或迷迭香酸溶液进行还原剂/抗氧化剂处理。两步自酸蚀粘接剂粘接，复合树脂修复，人工唾液保存24 h或1年。通过三因素ANOVA和事后Tukey检验和t检验（p < 0.05）分析微拉伸粘结强度（mu TBS）。透射电镜下观察杂交复合物中胶原纤维的状态。结果表明，保存1年后，龋坏牙本质的mu TBS显著降低（p < 0.05）。用NaOCl去除玷污层蛋白显著降低了龋损牙本质的mu TBS，尽管消除了杂交玷污层，但是随后应用对甲苯亚磺酸钠盐和迷迭香酸溶液显著增加了初始粘结强度（p < 0.05），并在储存1年后保持粘结强度（p > 0.05），在真正的混合层内保留了胶原-原纤维的完整性。因此，在用NaOCl进行玷污层脱蛋白后，额外应用对甲苯亚磺酸钠盐和迷迭香酸溶液可以提高自酸蚀粘合剂对受龋影响的牙本质的初始和长期粘结强度。
This study aimed to evaluate the effect of a reducing agent containingp-toluenesulfinic acid sodium salt (Accel (R)) and plant-extract antioxidant (rosmarinic acid solution) on the bonding durability of a self-etch adhesive to caries-affected dentin with and without smear layer-deproteinizing with NaOCl solution. The caries-affected dentin were assigned to pretreatment of smear layer-deproteinizing with NaOCl solution or none, then subdivided to reducing agent/antioxidant treatment with p-toluenesulfinic acid sodium salt or rosmarinic acid solution or none. After bonding with 2-step self-etch adhesive and restoring with resin composite, the specimens were stored in artificial saliva for 24 h or 1 year. The microtensile bond strength (mu TBS) were analyzed by threeway ANOVA with post-hoc Tukey's test and t-test (p < 0.05). The collagen-fibril status at hybridized complex was observed under TEM. The results showed that mu TBS to caries-affected dentin significantly decreased after 1-year of storage (p < 0.05). Smear layer-deproteinizing with NaOCl significantly decreased mu TBS to caries-affected dentin despite eliminating hybridized smear layer, however a subsequent application with p-toluenesulfinic acid sodium salt and rosmarinic acid solution significantly increased initial bond strengths (p < 0.05) and maintained the bond strengths after 1-year of storage (p > 0.05), with preservation of collagen-fibril integrity within authentic hybrid layer. Therefore, the additional application of p-toluenesulfinic acid sodium salt and rosmarinic acid solution after smear layer-deproteinizing with NaOCl could improve initial and long-term bond strengths of a self-etch adhesive to caries-affected dentin.