The acoustic spectrophonometer: a novel bioanalytical technique based on multifrequency acoustic devices.
The acoustic spectrophonometer: a novel bioanalytical technique based on multifrequency acoustic devices.
复制标题
声学分光计：一种基于多频声学装置的新型生物分析技术。
DOI：
10.1039/b307991k
复制
发表时间：
2003
期刊：
影响因子：
--
通讯作者：
C. Lowe
中科院分区：
文献类型：
--
作者：
A. Stevenson;B. Araya;R. S. Sethi;H. Mehta;C. Lowe
本文描述了一种类似于光学吸收光谱但基于倏逝声波的测量技术。这种格式采用平面螺旋线圈以 6 至 400 MHz 的频率振动石英单晶，以测量多频声谱。当应用于多个频率时，发现这些特定操作条件下测量值和计算值之间的显着拟合与定义的 Sauerbrey 和 Kanazawa 术语 K1 和 K2 一致：对于 13.5 ng mm(-2) 的 IgG 表面密度，K1 的测量值为 22.5 x 10(-6)，计算值为 20.4 x 10(-6)，而对于5.131 cP，K2的测量值为0.47，计算值为0.54。因此，对于这些特定的表面载荷，多频数据与 Sauerbrey 模型的预测吻合度在 10% 以内，与 Kanazawa 模型的预测吻合度在 13% 以内。然而，发现蔗糖、葡聚糖和葡萄糖的 5.131 cP 溶液的集体频移随频率表现出意想不到的额外变异性 (R2 < 0.4)，但在插值 K2 值的 2 倍内保留了频率依赖性的平方根。发现对 5.131 cP 葡聚糖溶液的响应明显低于其他等粘溶液，其频移和 K2 值比其本体粘度预期的显着降低。与这些粘性溶液相比，IgG 蛋白膜始终产生线性频移，几乎没有散射 (R2 > 0.96)，与工作频率成正比，并且与这些特定条件下的 Sauerbrey 模型完全一致。根据两组的方差和均值计算出 t 检验值为 14.52，表明声分光光度计可用于区分在单一工作频率下无法明显区分的两个化学系统的声阻抗特性。
A measurement technique similar to optical absorption spectroscopy but based on evanescent acoustic waves is described in this paper. This format employs a planar spiral coil to vibrate a single crystal of quartz from 6 to 400 MHz, in order to measure multifrequency acoustic spectra. Consistency with the defined Sauerbrey and Kanazawa terms K1 and K2 when applied to multiple frequencies was found for these specific operating conditions in terms of a significant fit between the measured and calculated values: For an IgG surface density of 13.5 ng mm(-2) the measured value of K1 is 22.5 x 10(-6) and the calculated value is 20.4 x 10(-6), whilst for glycerol viscous loadings of 5.131 cP the measured value of K2 is 0.47 and the calculated value is 0.54. Thus for these specific surface loadings the multifrequency data fits to the predictions of the Sauerbrey model to within 10% and to Kanazawa model within 13%. However collective frequency shifts for 5.131 cP solutions of sucrose, dextran and glucose were found to exhibit an unanticipated additional variability (R2 < 0.4) with frequency, but retained a square root of frequency dependency within a factor 2 of the interpolated K2 values. The response to the 5.131 cP dextran solution was found to be significantly below the other isoviscous solutions, with a substantially reduced frequency shift and K2 value than would be expected from its bulk viscosity. In comparison with these viscous solutions, IgG protein films consistently produced linear frequency shifts with little scatter (R2 > 0.96) that were proportional to the operating frequency, and fully consistent with the Sauerbrey model under these specific conditions. A t-test value of 14.52 was calculated from the variance and mean of the two groups, and demonstrates that the acoustic spectrophonometer can be used to distinguish between the acoustic impedance characteristics of two chemical systems that are not clearly differentiable at a single operating frequency.