OVARIAN NUTRITIONAL RESOURCES DURING THE REPRODUCTIVE CYCLE OF THE HEMATOPHAGOUS DIPETALOGASTER MAXIMA (HEMIPTERA: REDUVIIDAE): FOCUS ON LIPID METABOLISM
OVARIAN NUTRITIONAL RESOURCES DURING THE REPRODUCTIVE CYCLE OF THE HEMATOPHAGOUS DIPETALOGASTER MAXIMA (HEMIPTERA: REDUVIIDAE): FOCUS ON LIPID METABOLISM
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DOI：
10.1002/arch.21186
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发表时间：
2014-11-01
影响因子：
2.2
通讯作者：
Canavoso, Lilian E.
中科院分区：
文献类型：
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作者：
Leyria, Jimena;Fruttero, Leonardo L.;Canavoso, Lilian E.
在本研究中，我们分析了二瓣龙在生殖周期的代表性日子里卵巢营养资源的变化：卵黄发生前、卵黄发生以及禁食引起的早期和晚期闭锁。正如预期的那样，卵巢脂质、蛋白质和糖原的量从卵黄发生前到卵黄发生期间显着增加，然后在闭锁期间减少。然而，与卵黄发生前记录的相比，闭锁阶段发现的脂质和蛋白质储存量更高。卵巢组织切片的特异性脂质染色证明了整个生殖周期中脂滴的形状、大小和分布的显着变化。通过共注射 Lp-OG（其中 OG 是俄勒冈绿）和 Lp-DiI（其中 DiI 是 1,10-双十八烷基-3,3,30,30-四甲基吲哚碳花青）来分析脂蛋白 (Lp) 作为卵黄蛋白前体的作用，以跟踪整个颗粒，证明两种探针主要共定位于卵黄发生卵母细胞的卵黄体中。免疫荧光测定还表明，Lp 与卵黄体相关，支持其在卵黄发生过程中的内吞途径。通过共注射荧光探针来追踪整个颗粒 (Lp-DiI) 及其脂质货物 (Lp-Bodipy-FA) 的命运，在体内研究了 Lp 在向卵母细胞的脂质递送中的参与。 Lp-DiI 很容易被卵黄发生的卵母细胞吸收，并且在闭锁阶段的卵巢终末卵泡中没有观察到脂蛋白的摄取。 Bodipy-FA 迅速转移至卵黄发生卵母细胞，并在较小程度上转移至卵黄发生前卵泡和闭锁阶段卵巢组织的卵母细胞。 Lp-DiI 和 Lp-Bodipy-FA 在卵黄体内的共定位表明，Lp 在卵黄发生过程中卵母细胞脂质和蛋白质储存的积累中具有相关性。 (C) 2014 年 Wiley 期刊公司。
In this study, we have analyzed the changes of the ovarian nutritional resources in Dipetalogaster maxima at representative days of the reproductive cycle: previtellogenesis, vitellogenesis, as well as fasting-induced early and late atresia. As expected, the amounts of ovarian lipids, proteins, and glycogen increased significantly from previtellogenesis to vitellogenesis and then, diminished during atresia. However, lipids and protein stores found at the atretic stages were higher in comparison to those registered at previtellogenesis. Specific lipid staining of ovarian tissue sections evidenced remarkable changes in the shape, size, and distribution of lipid droplets throughout the reproductive cycle. The role of lipophorin (Lp) as a yolk protein precursor was analyzed by co-injecting Lp-OG (where OG is Oregon Green) and Lp-DiI (where DiI is 1,10-dioctadecyl-3,3,30,30-tetramethylindocarbocyanine) to follow the entire particle, demonstrating that both probes colocalized mainly in the yolk bodies of vitellogenic oocytes. Immunofluorescence assays also showed that Lp was associated to yolk bodies, supporting its endocytic pathway during vitellogenesis. The involvement of Lp in lipid delivery to oocytes was investigated in vivo by co-injecting fluorescent probes to follow the fate of the entire particle (Lp-DiI) and its lipid cargo (Lp-Bodipy-FA). Lp-DiI was readily incorporated by vitellogenic oocytes and no lipoprotein uptake was observed in terminal follicles of ovaries at atretic stages. Bodipy-FA was promptly transferred to vitellogenic oocytes and, to a much lesser extent, to previtellogenic follicles and to oocytes of ovarian tissue at atretic stages. Colocalization of Lp-DiI and Lp-Bodipy-FA inside yolk bodies indicated the relevance of Lp in the buildup of lipid and protein oocyte stores during vitellogenesis. (C) 2014 Wiley Periodicals, Inc.