Partial conservation of LFY function between rice and Arabidopsis
Partial conservation of LFY function between rice and Arabidopsis
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DOI：
10.1093/pcp/pcg155
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发表时间：
2003-12-01
影响因子：
4.9
通讯作者：
Kyozuka, J
中科院分区：
文献类型：
--
作者：
Chujo, A;Zhang, Z;Kyozuka, J
LFY/FLO基因编码植物特异性转录因子，并在生殖过渡和花卉发育中起主要作用。在这项研究中，我们重建了来自各种植物物种的49个LFY/FLO同源物的系统发育树。该树清楚地表明，来自Eudicot和Monocots的LFY/FLO基因。分别以非常高的自举概率形成了两个单系簇。此外，与Eudicot同源物相比，草LFY/FLO基因的氨基酸替代率显着加速。为了测试草LFY/FLO基因是否具有eudicots的功能保守的功能，我们将RFL引入了米型同源物RFL，将其引入了拟南芥LFY突变体中。由LFY启动子驱动的RFL基因部分挽救了LFY突变，这表明LFY和RFL的功能部分重叠。有趣的是，即使在营养组织中，RFL但不是LFY强烈激活了LFY的下游靶标的AP1和Ag的表达。 LFY :: RFL转基因拟南芥植物表现出异常的发育模式，例如叶卷曲，浓密的外观以及胚珠转化为皮层。所有结果表明，水稻和拟南芥之间LFY功能的部分保护和差异。
The LFY/FLO genes encode plant-specific transcription factors and play major roles in the reproductive transition as well as floral development. In this study, we reconstructed the phylogenetic tree of the 49 LFY/FLO homologs from various plant species. The tree clearly shows that the LFY/FLO genes from the eudicots and monocots; formed the two monophyletic clusters with very high bootstrap probabilities, respectively. Furthermore, grass LFY/FLO genes have experienced significant acceleration of amino acid replacement rate compared with the eudicot homolog. To test whether grass LFY/FLO genes have a conserved function with those of eudicots, we introduced RFL, a rice LFY homolog, into the Arabidopsis lfy mutant. The RFL gene driven by LFY promoter partially rescued the lfy mutation, suggesting that the functions of LFY and RFL partly overlap. Interestingly, the RFL but not LFY, strongly activated the expression of AP1 and AG, the downstream targets of LFY, even in the vegetative tissues. The LFY::RFL transgenic Arabidopsis plants exhibited abnormal patterns of development such as leaf curling, bushy appearance and the transformation of ovules into carpels. All of the results indicate that both the partial conservation and divergence of LFY function between rice and Arabidopsis.