Characterization of the nuclear polyhedrosis virus DNA of Adoxophyes orana and of Barathra brassicae.
Characterization of the nuclear polyhedrosis virus DNA of Adoxophyes orana and of Barathra brassicae.
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Adoxophyes orana 和 Barathra brasicae 核多角体病毒 DNA 的表征。
DOI：
10.1016/0042-6822(79)90271-x
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发表时间：
1979
期刊：
影响因子：
3.7
通讯作者：
J. Schegget
中科院分区：
文献类型：
--
作者：
M. Jurkovíčová;J. Touw;J. Sussenbach;J. Schegget
从橙黄夜蛾核型多角体病毒（NPV）和甘蓝夜蛾核型多角体病毒（NPV）中分离出环状双链DNA，并测定了它们的一些物理性质。A.奥兰多湾通过电镜和复性动力学分析，分别测定了6.7 × 107和8.9 × 107的BrassicaeNPV-DNA。后者的分析还表明，两个基因组都不包含重复序列。竞争杂交结果表明，两种病毒DNA之间无同源性。OranaNPV-DNA与B.油菜核型多角体病毒DNA用限制性内切酶EcoR Ⅰ对这些DNA进行分析，证实它们是不同的。CsCl中A. oranaNPV-DNA和B. BrassicaeNPV-DNA的含量分别为1.694和1.696 g/cm ~ 3，与热变性法测定的（G + C）含量分别为34.5%和37%一致。
Circular double-stranded DNA was isolated from nuclear polyhedrosis virus (NPV) ofAdoxophyes orana(virus particles singly embedded in the polyhedral matrix) and NPV ofBarathra brassicae(virus particles multiply embedded in the polyhedral matrix), and some of their physical properties were determined. The molecular weights ofA. oranaandB. brassicaeNPV-DNA, 6.7 x 107and 8.9 x 107, respectively, were determined by electron microscopy and by renaturation kinetics analysis. The latter analysis also showed that both genomes do not contain repetitive sequences. Absence of homology between DNA of these two viruses was shown by competition hybridization ofA. oranaNPV-DNA withB. brassicaeNPV-DNA. Analysis of these DNAs with the restriction endonucleaseEcoRI confirmed that they are different. The buoyant densities in CsCl ofA. oranaNPV-DNA and ofB. brassicaeNPV-DNA, 1.694 and 1.696 g/cm3, respectively, are consistent with (G + C) contents of 34.5 and 37%, respectively, as determined by thermal denaturation.