ENHANCED IMMUNE-RESPONSES IN BROILER CHICKS FED METHIONINE-SUPPLEMENTED DIETS
ENHANCED IMMUNE-RESPONSES IN BROILER CHICKS FED METHIONINE-SUPPLEMENTED DIETS
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DOI：
10.3382/ps.0661147
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发表时间：
1987-07-01
期刊：
影响因子：
4.4
通讯作者：
SUNDE, ML
中科院分区：
文献类型：
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作者：
TSIAGBE, VK;COOK, ME;SUNDE, ML
在玉米-豆粕型日粮中添加21%粗蛋白、3, 255 kcal代谢能/kg日粮、0.35%蛋氨酸、0.37%胱氨酸和0.13%胆碱，研究了蛋氨酸和胆碱对肉仔鸡生长和免疫功能的影响。蛋氨酸（0.063，0.125，0.25%）和胆碱（0.125，0.25%）是膳食变量。硫酸盐（0.055%）单独加入或与蛋氨酸（0.125或0.25%）和胆碱（0.125%）一起沿着加入。在一项研究中，0.25%蛋氨酸饮食补充有0.121%甜菜碱。钠和氯化物水平是恒定的，在所有的饮食。自由采食饲料和蒸馏水。用绵羊红细胞腹腔接种3周龄雏鸡，测定总抗体、免疫球蛋白（IG）G（2-巯基乙醇抗性抗体）和IgM（2-巯基乙醇敏感性抗体）。通过翅蹼肿胀评估胸腺衍生的（T）细胞依赖的体内有丝分裂原对植物血凝素-P（PHA-P）的反应。生长（0至3周龄）的蛋氨酸需求量约为0.413%的饮食（0.35%的基础饮食加0.063%）。基础日粮中添加0.125%的胆碱刺激生长的程度相同，额外的0.063%的蛋氨酸。添加0.055%的硫酸盐和0.125%的胆碱并不能提高后者保留蛋氨酸的能力。补充甲硫氨酸导致总抗体、IgG和对有丝分裂原PHA-P的反应的显著（P < 0.05）剂量相关性增加，但IgM没有增加。胆碱对研究的免疫变量没有影响。这些结果表明，甲硫氨酸是抗体反应的选择成分所必需的，这种作用可能与T细胞的帮助有关。
Effects of feeding supplementary methionine and choline on broiler growth and immunity were examined by supplementing a corn-soybean diet that contained 21% crude protein, 3,255 kcal metabolizable energy/kg diet, 0.35% methionine, 0.37% cystine, and 0.13% choline. Methionine (0.063, 0.125, 0.25%) and choline (0.125, 0.25%) were dietary variables. Sulfate (0.055%) was added either alone or along with methionine (0.125 or 0.25%) and choline (0.125%). In one study, the 0.25% methionine diet was supplemented with 0.121% betaine. Sodium and chloride levels were constant in all the diets. Feed and distilled water were supplied ad libitum. Total antibodies, immunoglobulin (Ig) G (2-mercaptoethanol-resistant antibodies) and IgM (2-mercaptoethanol-sensitive antibodies) were determined in 3-wk-old chicks inoculated intraperitoneally with sheep red blood cells. The thymus-derived (T)-cell-dependent in vivo mitogen response to phytohemagglutinin-P (PHA-P) was assessed via wing web swelling. The methionine requirement for growth (0 to 3 wk of age) was approximately 0.413% of the diet (0.35% in the basal diet plus 0.063% added). Supplementation of the basal diet with 0.125% choline stimulated growth to the same extent as did the extra 0.063% of methionine. Addition of 0.055% sulfate with 0.125% choline did not improve the ability of the latter to spare methionine. Supplemental methionine resulted in significant (P < 0.05) dose-related increases in total antibody, IgG, and response to the mitogen PHA-P, but not in IgM. There were no effects of choline on the immune variables studied. These results suggest that methionine is required for select components of the antibody response, which effect might be related to T-cell help.