Bloodstream cells phenotypically identical to human mesenchymal bone marrow stem cells circulate in large amounts under the influence of acute large skin damage:: New evidence for their use in regenerative medicine
Bloodstream cells phenotypically identical to human mesenchymal bone marrow stem cells circulate in large amounts under the influence of acute large skin damage:: New evidence for their use in regenerative medicine
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DOI：
10.1016/j.transproceed.2006.02.053
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发表时间：
2006-04-01
影响因子：
0.9
通讯作者：
Soratti, C.
中科院分区：
文献类型：
--
作者：
Mansilla, E.;Marin, G. H.;Soratti, C.
目标.最近的研究表明，人骨髓中含有间充质干细胞（MSC）。然而，很少有人知道他们在外周血中的存在。由于这些细胞可能负责损伤后的组织修复，因此在这些情况下应增加它们的数量。为了证明它们在这些情况下的数量，我们测量了健康供体和烧伤患者外周血中的MSC。采集15例急性烧伤患者和15例健康献血员的血样。我们使用大型单克隆抗体组进行流式细胞术分析：CD 44、CD 45、CD 14、DR、CD 34、CD 19、CD 13、CD 29、CD 105、CD 1a、CD 90、CD 38、CD 25。MSC表型被认为是CD 44、CD 13、CD 29、CD 90和CD 105阳性，而其他单克隆抗体阴性。在损伤后第3天进行测试。我们将结果与年龄、性别、烧伤的大小和类型进行了相关性分析。在两组的外周血中检测到表达MSC表型的细胞。值得注意的是，与来自健康供体的样本（0.0078 +/- 0.0044）相比，从烧伤患者获得的血液显示出更高的MSC百分比（0.1643 +/- 0.115; P <0.001）。MSCs的百分比与烧伤的大小和严重程度相关。在年轻患者中也观察到增加的值。MSC在人体组织的再生过程中具有重要作用。我们发现细胞表型相同的MSC循环在正常受试者的生理数量，但在急性大面积烧伤的显着更高的量。因此，它们可能代表了皮肤再生过程中以前未被认识到的循环成分。
Objectives. Recent work has shown that human bone marrow contains mesenchymal stem cells (MSCs). However, little is known about their presence in peripheral blood. Since these cells are potentially responsible for tissue repair after injury, their number should be increased during these situations. To demonstrate their number during these situations, we measured MSCs in the peripheral blood of healthy donors and burn patients.Materials and Methods. Blood samples were obtained from 15 acute burn patients and 15 healthy donors. We performed flow cytometric analysis, using a large monoclonal antibody panel: CD44, CD45, CD14, DR, CD34, CD19, CD13, CD29, CD105, CD1a, CD90, CD38, CD25. MSC phenotype was considered positive for CD44, CD13, CD29, CD90, and CD105, and negative for the other monoclonals. The testing was performed on day 3 after injury. We correlated the results with the age, sex, and size and type of burns.Results. Cells expressing the MSC phenotype were detected in the peripheral blood of both groups. Noteworthy, compared with samples from healthy donors (0.0078 +/- 0.0044), blood obtained from burn patients showed a higher MSC percentage (0.1643 +/- 0.115; P < .001). The percentage of MSCs correlated with the size and severity of the burn. Increased values were also observed among younger patients.Conclusions. MSCs have an important role in regenerative processes of human tissues. We found cells phenotypically identical to MSCs circulating in physiological number in normal subjects, but in significantly higher amounts during acute large burns. Therefore, they may represent a previously unrecognized circulatory component to the process of skin regeneration.