EVIDENCE FOR RELEASE OF VASOPRESSIN AND OXYTOCIN INTO CEREBROSPINAL-FLUID - MEASUREMENTS IN PLASMA AND CSF OF INTACT AND HYPOPHYSECTOMIZED RATS
EVIDENCE FOR RELEASE OF VASOPRESSIN AND OXYTOCIN INTO CEREBROSPINAL-FLUID - MEASUREMENTS IN PLASMA AND CSF OF INTACT AND HYPOPHYSECTOMIZED RATS
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DOI：
10.1159/000122702
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发表时间：
1977-01-01
影响因子：
4.1
通讯作者：
SWAAB, DF
中科院分区：
文献类型：
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作者：
DOGTEROM, J;VANWIMERSMAGREIDANUS, TB;SWAAB, DF
用放射免疫法测定不同条件下Wistar大鼠脑脊液和血浆中精氨酸-8-加压素(AVP)和催产素(OXT)的含量。测定Brattleboro大鼠的基础血浆OXT水平，无论是纯合子的还是杂合子的尿崩症(分别为HO-DI或He-DI)。与He-DI和Wistar大鼠相比，HO-DI大鼠基础OXT水平升高。缺水导致Wistar大鼠血浆OXT水平逐渐升高。脑脊液取自Wistar大鼠，在戊巴比妥钠麻醉下从枕大池取出。麻醉诱导血浆AVP和OXT水平升高。停用脑脊液后，血浆AVP水平显著升高，OXT水平略有升高。在被研究的大鼠中，在垂体切除后4wk，血浆AVP水平只有在脑脊液退出后才能检测到。这些动物的脑脊液中AVP水平升高。在正常大鼠和去垂体大鼠，基础OXT血浆水平和麻醉后升高的水平相同。脑脊液撤除后，OXT进入血液的释放部分受损，因为撤除脑脊液不会导致血浆OXT水平的进一步升高。正常大鼠和去垂体大鼠的脑脊液催产素水平相同。脑脊液和血浆中AVP的含量不同，而OXT的含量不同。AVP和OXT纤维的再生方式不同，导致激素释放到脑脊液和血浆中的方式不同。
Arginine-8-vasopressin (AVP) and oxytocin (OXT) were measured by radioimmunoassay (RIA) in cerebrospinal fluid (CSF) and plasma of Wistar rats under various conditions. Basal plasma levels of OXT were measured in Brattleboro rats, either homozygous or heterozygous for diabetes insipidus (Ho-DI or He-DI respectively). The basal OXT plasma level of Ho-DI rats was elevated compared to He-DI and Wistar rats. Water deprivation caused a gradual increase of plasma OXT levels in Wistar rats. CSF was collected from Wistar rats by withdrawal from the cisterna magna under pentobarbitone anesthesia. Anesthesia induced a rise of the plasma AVP level and of the plasma OXT level. Withdrawal of CSF caused a dramatic rise of plasma AVP levels and, to a minor extent, of plasma OXT levels. In rats studied at 4 wk after hypophysectomy plasma AVP levels were only detectable after CSF withdrawal. CSF levels of AVP were elevated in these animals. In intact and hypophysectomized rats, basal OXT plasma levels and the increased levels after anesthesia were the same. The OXT release into the blood was partly impaired after hypophysectomy because CSF withdrawal did not result in a further rise of plasma OXT levels. CSF oxytocin levels were the same in intact and hypophysectomized rats. A difference of the effect of hypophysectomy was observed with respect to the presence in CSF and plasma for AVP but not for OXT. The regeneration of AVP and OXT fibers is different which leads to a different release of the hormones into CSF and plasma.