Molecularly imprinted polymer solid-phase extraction of synthetic cathinones from urine and whole blood samples
Molecularly imprinted polymer solid-phase extraction of synthetic cathinones from urine and whole blood samples
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DOI：
10.1002/jssc.201800874
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发表时间：
2018-12-01
影响因子：
3.1
通讯作者：
Hasegawa, Hiroshi
中科院分区：
文献类型：
--
作者：
Murakami, Takaya;Iwamuro, Yoshiaki;Hasegawa, Hiroshi
在法医药物分析中，萃取预处理需要在仪器分析之前进行，以确保目标化合物的成功检测。然而，传统的萃取方法，如亲水聚合物固相萃取和液-液萃取，不适合一类新兴的新型精神活性物质，即合成卡西酮，因为它们缺乏类选择性，并且在提取过程中目标分析物分解的风险增加。为了解决这些问题，我们描述了一种高度分类选择性的样品清理方法，用于从尿液和全血样本中提取合成卡西酮，利用分子印迹聚合物固相萃取盒。对于合成卡西酮分子结构对萃取回收率的影响，我们发现较长的烷基侧链会略微降低萃取效率，而芳香环上取代基的变化不会产生影响。分子印迹聚合物固相萃取尿液样品中11种合成卡西酮的回收率高于两种常规萃取方法，基质效应小于亲水性聚合物固相萃取方法。分子印迹聚合物固相萃取全血样品的回收率与尿液样品相当。因此，该方法适用于生物样品中合成卡西酮的提取和定量测定。
In forensic drug analysis, extractive pretreatment is required prior to instrumental analysis to ensure successful detection of the target compounds. However, conventional extraction methods such as hydrophilic polymer-based solid-phase extraction and liquid-liquid extraction are unsuitable for an emerging class of new psychoactive substances, namely, synthetic cathinones, because they exhibit a lack of class selectivity and increased risk of target analyte decomposition during extraction. To address these issues, we describe a highly class-selective sample clean-up method for the extraction of synthetic cathinones from urine and whole blood samples, exploiting a molecularly imprinted polymer solid-phase extraction cartridge. In terms of the influence of the synthetic cathinone molecular structure on the extraction recovery, we showed that while longer alkyl side chains slightly reduced the extraction efficiency, substituent variation on the aromatic ring exerted no effect. Molecularly imprinted polymer solid-phase extraction of 11 synthetic cathinones from urine samples yielded higher recoveries than the two conventional extraction methods, and smaller matrix effect was observed than that with hydrophilic polymer-based solid-phase extraction. Molecularly imprinted polymer solid-phase extraction from whole blood samples gave recoveries comparable to those of urine samples. Therefore, the proposed method is applicable for the extraction and quantitative determination of synthetic cathinones in biological samples.