Comparative evaluation of size-exclusion chromatography and viscometry for the characterisation of cellulose
Comparative evaluation of size-exclusion chromatography and viscometry for the characterisation of cellulose
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DOI：
10.1016/j.chroma.2003.11.002
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发表时间：
2004-02-13
影响因子：
4.1
通讯作者：
Mortha, G
中科院分区：
文献类型：
--
作者：
Dupont, AL;Mortha, G
使用尺寸排阻色谱法（SEC）与多角度光散射（MALS）和差示折射率（DRI）检测溶解在氯化锂/N-二甲基乙酰胺（LiCl/DMAc）的纤维素的分析进行了评估，并与目前用于纤维素分析的其他两种方法进行了比较。这些是SEC与低角度光散射（LALS）和紫外检测的纤维素衍生的三氨基甲酸酯（CTC），和粘滞素在镉三乙烯二胺二氢氧化物（cadoxen）。纤维素来源是Whatman No.1纸，未老化或人工老化的热和湿度的组合。用不同方法测得的老化纸和未老化纸的纤维素摩尔质量（M-r）平均值有很大差异。在LiCl/DMAc中纤维素的SEC提供了最高的M-r平均值，其次是CTC的SEC，而粘度计产生最低的值。这些差异或多或少地取决于纸张的初始降解状态而显著。为了解释这些差异，提出了几种假设，并讨论了每种方法的基础上，其适用性的老化引起的退化。(C)2003 Elsevier B. V.保留所有权利。
The analysis using size-exclusion chromatography (SEC) with multi-angle light scattering (MALS) and differential refractive index (DRI) detection of cellulose dissolved in lithium chloride/NN-dimethylacetamide (LiCl/DMAc) is evaluated and compared to two other methods currently used for cellulose analysis. These are SEC with low-angle light scattering (LALS) and ultra-violet detection of cellulose derivatised to tricarbanilates (CTC), and viscometry in cadmium triethylene diamine dihydroxide (cadoxen). The cellulose source is Whatman No. 1 paper, unaged or artificially aged with a combination of heat and humidity. The values of the molar mass (M-r) averages of cellulose obtained with the different methods resulted quite different for both aged and unaged paper. SEC of cellulose in LiCl/DMAc provided the highest M-r averages values, followed by SEC of CTC, while viscometry yielded the lowest values. These differences were more or less pronounced depending on the initial degradation state of the paper. Several hypotheses are presented in order to explain these discrepancies and each method is discussed on the basis of its suitability to characterise the aging-induced degradation. (C) 2003 Elsevier B.V. All rights reserved.