P elements and the determinants of hybrid dysgenesis have different dynamics of propagation in Drosophila melanogaster populations
P elements and the determinants of hybrid dysgenesis have different dynamics of propagation in Drosophila melanogaster populations
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DOI：
10.1007/s10709-015-9872-z
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发表时间：
2015-12-01
期刊：
影响因子：
1.5
通讯作者：
Fedorova, Svetlana A.
中科院分区：
文献类型：
--
作者：
Ignatenko, Olesia M.;Zakharenko, Lyudmila P.;Fedorova, Svetlana A.
某些黑腹果蝇品系杂交后会出现种内杂种发育不全（HD）。主要观点是转座P元件的运动导致HD。据信，P元件在上世纪中叶通过水平转移出现在黑腹果蝇基因组中，同时与HD决定簇的出现。随后发生了 HD 决定簇和 P 元件的同时扩展。我们分析了 HD 决定因素在黑腹果蝇自然种群中的当前分布，没有发现其进一步传播的证据。然而，在所有分析的天然黑腹果蝇菌株的基因组中都鉴定出了全尺寸的 P 元件，与其细胞型无关。因此，P 元素的扩展与 HD 决定因素的扩展不相关。我们发现，尽管在发育不良和非发育不良胚胎的早期阶段原始生殖细胞数量相同，但发育不良雌性的卵巢不含生殖细胞。我们认为 HD 不是由 P 元件转座引起的 DNA 损伤引起的，但可能是发育不良卵巢形成调节的破坏导致了发育不良表型。
Intraspecific hybrid dysgenesis (HD) appears after some strains of D. melanogaster are crossed. The predominant idea is that the movement of transposable P elements causes HD. It is believed that P elements appeared in the D. melanogaster genome in the middle of the last century by horizontal transfer, simultaneously with the appearance of HD determinants. A subsequent simultaneous expansion of HD determinants and P elements occurred. We analyzed the current distribution of HD determinants in natural populations of D. melanogaster and found no evidence of their further spread. However, full-sized P elements were identified in the genomes of all analyzed natural D. melanogaster strains independent of their cytotypes. Thus, the expansion of P elements does not correlate with the expansion of HD determinants. We found that the ovaries of dysgenic females did not contain germ cells despite the equal number of primordial germ cells in early stages in dysgenic and non-dysgenic embryos. We propose that HD does not result from DNA damage caused by P element transposition, but it would be the disruption in the regulation of dysgenic ovarian formation that causes the dysgenic phenotypes.