Development of a highly responsive needle-type glucose sensor using polyimide for a wearable artificial endocrine pancreas
Development of a highly responsive needle-type glucose sensor using polyimide for a wearable artificial endocrine pancreas
复制标题
DOI：
10.1007/s10047-005-0326-8
复制
发表时间：
2006-06
影响因子：
1.3
通讯作者：
S. Ichimori;K. Nishida;S. Shimoda;T. Sekigami;Y. Matsuo;K. Ichinose;M. Shichiri;M. Sakakida;E. Araki
中科院分区：
文献类型：
--
作者：
S. Ichimori;K. Nishida;S. Shimoda;T. Sekigami;Y. Matsuo;K. Ichinose;M. Shichiri;M. Sakakida;E. Araki
为了研制一种用于可穿戴式人工内分泌胰腺的长寿命、稳定、小型的葡萄糖传感器，我们研制了一种新型的聚酰亚胺微针式葡萄糖传感器，命名为PI传感器(外径0.3 mm，长度16 mm)，并对其体内外特性进行了研究。在体外研究中，我们在含有不同葡萄糖浓度的0.9%氯化钠溶液中对传感器进行了测试，观察到传感器的输出与葡萄糖浓度呈良好的线性关系(范围：0-500 mg/100 ml)。在体内实验中，将PI传感器植入Beagle犬的腹部皮下组织(n=5)，并在传感器校准后监测间质液体葡萄糖浓度。同时，静脉内放置另一个PI传感器，连续监测血糖浓度。皮下组织血糖(Y)与血糖浓度(X：30~350 mg/100 m l)的相关系数和时间延迟分别为y=1.03X+7.98(r=0.969)和6.6±1.2 m in。我们应用了新的WAEP系统/PI传感器和以前开发的静脉胰岛素输注算法来控制糖尿病狗的血糖。在口服1.5 g/kg血糖(挑战后血糖水平：65 时为176±18 mg/100 m l，240时为93±23 mg/100 m l)后，WAEP系统的血糖控制良好，且未出现任何低血糖。因此，我们证实了我们的新型PI传感器在体外和体内都具有良好的传感器特性。使用该传感器的新型WAEP具有潜在的临床应用价值。
To produce a long-life, stable, miniature glucose sensor for a wearable artificial endocrine pancreas (WAEP), we developed a novel microneedle-type glucose sensor using polyimide, designated the PI sensor (outer diameter, 0.3 mm; length, 16 mm), and investigated its characteristics in vitro and in vivo. In the in vitro study, we tested the sensor in 0.9% NaCl solution with varying glucose concentrations and observed an excellent linear relationship between the sensor output and glucose concentration (range: 0–500 mg/100 ml). In in vivo experiments, the PI sensor was inserted into the abdominal subcutaneous tissue of beagle dogs (n= 5), and interstitial fluid glucose concentrations were monitored after sensor calibration. Simultaneously, blood glucose concentrations were also monitored continuously with another PI sensor placed intravenously. The correlation and time delay between subcutaneous tissue glucose (Y) and blood glucose concentrations (X: 30–350 mg/100 ml) wereY= 1.03X+ 7.98 (r= 0.969) and 6.6 ± 1.2 min, respectively. We applied the new WAEP system/PI sensor and an intravenous insulin infusion algorithm developed previously for glycemic control in diabetic dogs. The use of the WAEP system resulted in excellent glycemic control after an oral glucose challenge of 1.5 g/kg (post-challenge blood glucose levels: 176 ± 18 mg/100 ml at 65 min and 93 ± 23 mg/100 ml at 240 min), without any hypoglycemia. Thus, we confirmed that our new PI sensor has excellent sensor characteristics in vitro and in vivo. The new WAEP using this sensor is potentially suitable for clinical application.