Comparison of liquid based cytology and histology for the evaluation of HER-2 status using immunostaining and CISH in breast carcinoma
Comparison of liquid based cytology and histology for the evaluation of HER-2 status using immunostaining and CISH in breast carcinoma
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DOI：
10.1136/jcp.2004.024224
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发表时间：
2005-08-01
影响因子：
3.4
通讯作者：
Birembaut, P
中科院分区：
文献类型：
--
作者：
Sartelet, H;Lagonotte, E;Birembaut, P
背景：HER- 2扩增是乳腺癌重要的预后生物标志物和治疗决定因素。目的：将HER- 2的免疫细胞化学（ICC）表达和用液体细胞学（LBC）原位显色杂交（CISH）测定的基因扩增与使用同种乳腺癌组织样本的免疫组织化学（IHC）和CISH相关联。方法：对103例乳腺癌的冰冻切片和细胞刷片进行分析。对每个肿瘤进行了四项技术：两项针对LBC样本（ICC和CISH，均分为阳性、不确定或阴性），两项针对组织学样本（IHC和CISH）。2株细胞系（MCF- 7，阴性；BT 474，阳性）作为对照进行细胞学分析。采用互补荧光原位杂交技术对组织学样品进行低扩增（4 - 10个点/细胞核）。结果：通过kappa系数计算的四种技术的观察者间协议表明了实质性的协议。9例细胞学检查因细胞性差而失败。94例中扩增19例；IHC评分为0或1+、2+和3+的分别为73、12和9个，ICC评分为75、13和6个。CISH在72个肿瘤中未发现扩增。组织学和细胞学样本的IHC和CISH结果之间的相关性总是显著的。结论：利用原位杂交技术可以确定LBC样本中Her- 2的状态，并与参考组织学方法具有良好的相关性。由于细胞质膜的存在，ICC不太可靠。然而，这些结果应该得到大型多中心研究的证实。
Background: HER- 2 amplification is an important prognostic biomarker and treatment determinant in breast carcinoma.Aims: To correlate immunocytochemical ( ICC) expression of HER- 2 and gene amplification determined by chromogenic in situ hybridisation ( CISH) using liquid based cytology ( LBC) with immunohistochemistry ( IHC) and CISH using histological samples of the same breast carcinomas.Methods: Frozen sections and cytobrushings of 103 breast carcinomas were analysed. Four techniques were performed on each tumour: two on LBC samples ( ICC, and CISH, both graded as positive, indeterminate, or negative) and two on histological samples ( IHC and CISH). Two cell lines ( MCF- 7, negative; BT 474, positive) were used as controls for cytological analysis. A complementary fluorescence in situ hybridisation technique was carried out in histological samples with low amplification ( 4 - 10 dots/ nucleus).Results: Interobserver agreement for the four techniques calculated by the kappa coefficient indicated a substantial agreement. Nine cases failed in cytology because of poor cellularity. Among 94 cases, 19 were amplified; 73, 12, and 9 tumours were scored 0 or 1+, 2+, and 3+, respectively by IHC and 75, 13, and 6, respectively, by ICC. CISH found no amplification in 72 tumours. Correlations between the IHC and CISH results in the histological and cytological samples were always significant.Conclusions: Her- 2 status could be determined in LBC samples and correlated well with reference histological methods using in situ hybridisation. ICC was less reliable because of the presence of the cytoplasmic membrane. However, these results should be confirmed by a large multicentre study.