Phospholipid exchange between restricted and nonrestricted domains in sarcoplasmic reticulum vesicles.
Phospholipid exchange between restricted and nonrestricted domains in sarcoplasmic reticulum vesicles.
复制标题
肌浆网囊泡中限制性结构域和非限制性结构域之间的磷脂交换。
DOI：
10.1016/0005-2736(85)90342-6
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Yeagle,PL
中科院分区：
文献类型：
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作者：
Selinsky,BS;Yeagle,PL
兔肌肉轻质肌浆网膜的磷核磁共振波谱由两个重叠的共振组成，一个比另一个宽得多。广泛的共振产生于磷脂的运动受限，可能与钙-ATPase有关，而窄的共振产生于磷脂仅被蛋白质的存在轻微扰动。(Selinsky，B.S.和Yeagle，P.L.(1984)生物化学23,2281-2288)。通过测量从宽共振到窄共振的磁化转移来确定由共振表示的两个磷脂结构域之间的交换速率。磷脂从限制性结构域到非限制性结构域的交换速率为1 S−1。
Phosphorus nuclear magnetic resonance spectra of rabbit muscle light sarcoplasmic reticulum membranes consist of two overlapping resonances, one much broader than the other. The broad resonance arises from phospholipids motionally restricted, probably by association with the Ca2+-ATPase, while the narrow resonance arises from phospholipid only slightly perturbed by the presence of the protein. (Selinsky, B.S. and Yeagle, P.L. (1984) Biochemistry 23, 2281–2288). The rate of exchange between the two phospholipid domains represented by the resonances was determined by measuring the transfer of magnetization from the broad resonance to the narrow resonance. The rate of exchange of phospholipids from the restricted domain to the nonrestricted domain was determined to be 1 s−1.