Novel Polypyrrole-Coated Polylactide Scaffolds Enhance Adipose Stem Cell Proliferation and Early Osteogenic Differentiation
Novel Polypyrrole-Coated Polylactide Scaffolds Enhance Adipose Stem Cell Proliferation and Early Osteogenic Differentiation
复制标题
DOI：
10.1089/ten.tea.2012.0111
复制
发表时间：
2013-04-01
影响因子：
4.1
通讯作者：
Haimi, Suvi
中科院分区：
文献类型：
--
作者：
Pelto, Jani;Bjorninen, Miina;Haimi, Suvi
掺杂生物活性剂的导电聚吡咯（PPy）是一种新兴的组织工程功能生物材料。因此，我们使用硫酸软骨素（CS）掺杂的 PPy 涂层来修饰最初的电绝缘聚丙交酯，从而产生新型成骨支架。采用原位化学氧化聚合在聚 96L/4D-丙交酯 (PLA) 非织造支架上获得导电 PPy 涂层。对涂层支架进行了表征，并在水解过程中评估了它们的电导率。将涂层导电支架在三维（3D）几何形状的电刺激（ES）下增强人类脂肪干细胞（hASC）增殖和成骨分化的能力与未涂层PLA支架进行比较。 PPy 涂层的 PLA 支架 (PLA-PPy) 的电导率在水解开始时很明显，但在孵育第一周由于去掺杂而下降。在第 7 天和第 14 天，与普通 PLA 支架相比，PLA-PPy 支架显着增强了 hASC 增殖。此外，在 PLA-PPy 接种的支架中，hASC 的碱性磷酸酶 (ALP) 活性通常较高，但由于患者差异，无法确定统计学显着性。 ES 对 hASC 没有显着影响。这项研究凸显了新型 PPy 涂层 PLA 支架在骨组织工程中的潜力。
An electrically conductive polypyrrole (PPy) doped with a bioactive agent is an emerging functional biomaterial for tissue engineering. We therefore used chondroitin sulfate (CS)-doped PPy coating to modify initially electrically insulating polylactide resulting in novel osteogenic scaffolds. In situ chemical oxidative polymerization was used to obtain electrically conductive PPy coating on poly-96L/4D-lactide (PLA) nonwoven scaffolds. The coated scaffolds were characterized and their electrical conductivity was evaluated in hydrolysis. The ability of the coated and conductive scaffolds to enhance proliferation and osteogenic differentiation of human adipose stem cells (hASCs) under electrical stimulation (ES) in three-dimensional (3D) geometry was compared to the noncoated PLA scaffolds. Electrical conductivity of PPy-coated PLA scaffolds (PLA-PPy) was evident at the beginning of hydrolysis, but decreased during the first week of incubation due to de-doping. PLA-PPy scaffolds enhanced hASC proliferation significantly compared to the plain PLA scaffolds at 7 and 14 days. Furthermore, the alkaline phosphatase (ALP) activity of the hASCs was generally higher in PLA-PPy seeded scaffolds, but due to patient variation, no statistical significance could be determined. ES did not have a significant effect on hASCs. This study highlights the potential of novel PPy-coated PLA scaffolds in bone tissue engineering.