Effect of Radix Ginseng and Radix Trichosanthis on the melanogenesis
Effect of Radix Ginseng and Radix Trichosanthis on the melanogenesis
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DOI：
10.1248/bpb.26.849
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发表时间：
2003-06-01
影响因子：
2
通讯作者：
Woo, WH
中科院分区：
文献类型：
--
作者：
Im, SJ;Kim, KN;Woo, WH
黑素生成是暴露于紫外线、遗传原因和其他来源的人类皮肤的众所周知的生理反应。本研究旨在探讨人参和天花粉对B16黑色素瘤细胞黑素合成的影响。将细胞用浓度范围为1至50 μ g/ml的RT、浓度为10-1000 μ g/ml的RG或不同剂量（10-1000 μ g/ml）的RG与25 μ g/ml RT处理48小时。与未处理的对照相比，单独用RT处理剂量依赖性地抑制酪氨酸酶活性和黑色素含量，并显著抑制细胞增殖。而不同浓度的RG对上述指标无明显影响。在各种浓度的RG的存在下用RT处理抑制酪氨酸酶活性和黑色素含量，类似于单独用RT处理，但略微增加细胞增殖。此外，酪氨酸酶蛋白水平显着降低，在治疗与25 μ g/ml RT单独和与100 μ g/ml RG的组合。这些结果表明，用RG和RT处理显著抑制B16细胞中的黑素生成，并提高了这种组合在皮肤增白剂中有效的可能性。
Melanogenesis is a well known physiological response of human skin exposed to ultraviolet light, genetic reasons and other sources. In this study, we conducted to evaluate the effects of Radix Ginseng (RG) and Radix Trichosanthis (RT) on the melanogenesis in the B16 melanoma cells. The cells were treated for 48 h with RT at concentrations ranging from 1 to 50 mug/ml, RG at concentration of 10-1000 mug/ml, or RG at various doses (10-1000 mug/ml) with 25 mug/ml RT. Treatment with RT alone dose-dependently suppressed tyrosinase activity and melanin content compared with untreated control, and significantly inhibited cell proliferation. However, RG at various concentrations did not exhibit any significant change of them. Treatment with RT in the presence of various concentrations of RG suppressed tyrosinase activity and melanin content, similar to treatment with RT alone, but slightly increased cell proliferation. Furthermore, tyrosinase protein level was significantly decreased in treatment with 25 mug/ml RT alone and with a combination of 100 mug/ml RG. These results indicate that treatment with RG and RT significantly inhibits the melanogenesis in B16 cells, and raise the possibility that this combination may be effective in the whitening agent for the skin.