Interaction of 2,3,7,8-tetrachlorodibenzo-p-dioxin and retinoic acid in MCF-7 human breast cancer cells.
Interaction of 2,3,7,8-tetrachlorodibenzo-p-dioxin and retinoic acid in MCF-7 human breast cancer cells.
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2,3,7,8-四氯二苯并-对二恶英和视黄酸在 MCF-7 人乳腺癌细胞中的相互作用。
DOI：
10.1006/taap.1994.1132
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发表时间：
1994
影响因子：
3.8
通讯作者：
Safe,S
中科院分区：
文献类型：
--
作者：
Lu,Y;Wang,X;Safe,S
视黄酸 (RA) 和 2,3,7,8-四氯二苯并-对二恶英 (TCDD) 都会引起许多常见的生化和毒性反应，包括对小鼠的致畸作用和抑制多种细胞系的细胞增殖。在小鼠中，RA 和 TCDD 作为致畸剂协同相互作用，表明 RA 和芳烃受体信号转导途径之间存在联系。本研究表明，RA 和 TCDD 在 MCF-7 人乳腺癌细胞中引发许多常见反应，包括抑制雌激素诱导的细胞增殖和 [3H] 胸苷摄取、抑制核雌激素受体 (ER) 配体结合，以及在凝胶迁移率变化测定中与共有的 [32] 雌激素反应元件相互作用。 RA 和 TCDD 也会以时间依赖性方式降低稳态 ER mRNA 水平。此外，用 RA 加 TCDD 处理细胞的交互式研究也导致反应下降。
Both retinoic acid (RA) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) elicit a number of common biochemical and toxic responses including teratogenic effects in mice and inhibition of cell proliferation of numerous cell lines. In mice, RA plus TCDD interact synergistically as teratogens, suggesting a link between the RA and aryl hydrocarbon receptor signal transduction pathways. In this study, it was shown that both RA and TCDD elicit a number of common responses in MCF-7 human breast cancer cells, including inhibition of estrogen-induced cell proliferation and [3H]thymidine uptake, inhibition of nuclear estrogen receptor (ER) ligand binding, and interactions with a consensus [32]estrogen-responsive element in a gel mobility shift assay. RA and TCDD also decrease steady-state ER mRNA levels in a time-dependent manner. Moreover, interactive studies in which cells were treated with both RA plus TCDD also resulted in decreased responses.