Identification of candidate genes of QTLs for seed weight in Brassica napus through comparative mapping among Arabidopsis and Brassica species.
Identification of candidate genes of QTLs for seed weight in Brassica napus through comparative mapping among Arabidopsis and Brassica species.
复制标题
通过拟南芥和芸苔属植物比较作图鉴定甘蓝型油菜种子重量 QTL 候选基因
DOI：
10.1186/1471-2156-13-105
复制
发表时间：
2012-12-06
期刊：
影响因子：
2.9
通讯作者：
Zhou Y
中科院分区：
文献类型：
--
作者：
Cai G;Yang Q;Yang Q;Zhao Z;Chen H;Wu J;Fan C;Zhou Y
背景
由于多倍体作物基因组结构的复杂性，其数量性状基因座（QTL）的图位克隆仍然是一个挑战。B中粒重的QTL。napus的QTL，但这一重要性状的候选基因的信息仍然很少。
结果
本研究构建了B.利用SSR标记构建了一个甘蓝型油菜的遗传距离为2126.4cM的SSR标记，标记间的平均距离为5.36cM。建立了B上SSR位点共线性的方法。napus及其两个祖先二倍体物种B。rapa和B. oleracea通过广泛的生物信息学分析。在B的帮助下。rapa和B.甘蓝基因组序列中，由B的SSR位点推导出421个同源共线性位点。napus中的398个同源位点对应于拟南芥中的398个同源位点。通过对拟南芥和芸苔属3种植物的比较定位，将227个与种子大小/重量相关的同源基因定位在B区。建立了甘蓝型油菜遗传图谱，为甘蓝型油菜重要农艺性状的遗传基础奠定了基础。利用B区的种子重/粒重基因分布图，筛选出8个QTL，共12个候选基因，构建了BnAP 2的分子标记。油菜。
结论
本研究表明，利用SSR标记B技术检测QTL候选基因是可行的。通过拟南芥和B的比较作图构建了甘蓝型油菜遗传图谱。napus及其两个祖先种B. rapa和B.甘蓝。双二倍体B种子重候选基因的鉴定。napus的QTL定位将加快这一重要性状QTL定位的进程，该方法可用于其他农艺性状的QTL定位。
Background
Map-based cloning of quantitative trait loci (QTLs) in polyploidy crop species remains a challenge due to the complexity of their genome structures. QTLs for seed weight in B. napus have been identified, but information on candidate genes for identified QTLs of this important trait is still rare.
Results
In this study, a whole genome genetic linkage map for B. napus was constructed using simple sequence repeat (SSR) markers that covered a genetic distance of 2,126.4 cM with an average distance of 5.36 cM between markers. A procedure was developed to establish colinearity of SSR loci on B. napus with its two progenitor diploid species B. rapa and B. oleracea through extensive bioinformatics analysis. With the aid of B. rapa and B. oleracea genome sequences, the 421 homologous colinear loci deduced from the SSR loci of B. napus were shown to correspond to 398 homologous loci in Arabidopsis thaliana. Through comparative mapping of Arabidopsis and the three Brassica species, 227 homologous genes for seed size/weight were mapped on the B. napus genetic map, establishing the genetic bases for the important agronomic trait in this amphidiploid species. Furthermore, 12 candidate genes underlying 8 QTLs for seed weight were identified, and a gene-specific marker for BnAP2 was developed through molecular cloning using the seed weight/size gene distribution map in B. napus.
Conclusions
Our study showed that it is feasible to identify candidate genes of QTLs using a SSR-based B. napus genetic map through comparative mapping among Arabidopsis and B. napus and its two progenitor species B. rapa and B. oleracea. Identification of candidate genes for seed weight in amphidiploid B. napus will accelerate the process of isolating the mapped QTLs for this important trait, and this approach may be useful for QTL identification of other traits of agronomic significance.