Ubiquitin extension protein UEP1 modulates cell death and resistance to various pathogens in tobacco
Ubiquitin extension protein UEP1 modulates cell death and resistance to various pathogens in tobacco
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泛素延伸蛋白 UEP1 调节烟草中的细胞死亡和对各种病原体的抵抗力
DOI：
10.1094/phyto-06-18-0212-r
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发表时间：
2019
期刊：
影响因子：
3.2
通讯作者：
Cai XZ
中科院分区：
文献类型：
--
作者：
Xu YP;Zhao Y;Song XY;Ye YF;Wang RG;Wang ZL;Ren XL;Cai XZ
泛素 (Ub) 延伸蛋白 (UEP) 是 N 末端 Ub 与核糖体蛋白的融合蛋白。它们是泛素的主要来源，也是延伸核糖体蛋白的唯一来源。尽管Ub-26S蛋白酶体系统在各种生物过程中的重要作用已得到充分证实，但UEP基因在植物防御中作用的直接证据却很少报道。在本研究中，我们从本塞姆氏烟草中克隆了一个Ub-S27a型UEP基因（NbUEP1），并证明了其在细胞死亡和抗病性中的功能。病毒诱导的 NbUEP1 基因沉默导致大量细胞死亡，最终导致整株幼苗枯萎。 NbUEP1的瞬时RNA干扰(RNAi)导致渗透区域细胞强烈死亡，而稳定的NbUEP1-RNAi烟草植株在叶片中组成性地形成坏死病斑。与非RNAi相比，NbUEP1-RNAi植株对卵菌腐霉和病毒烟草花叶病毒和黄瓜花叶病毒的抵抗力增强，而对线虫根结线虫的抵抗力下降控制植物。转录谱分析表明，茉莉酸和乙烯途径、脂质代谢、铜胺氧化酶介导的活性物质生成、富含甘氨酸的蛋白质、液泡加工酶和RD21介导的细胞死亡和防御调节以及自噬可能与NbUEP1介导的细胞死亡和抵抗相关。我们的结果为植物 UEP 在调节植物细胞死亡和抗病性方面的重要作用提供了证据。
Ubiquitin (Ub) extension proteins (UEPs) are fusion proteins of a Ub at the N terminus to a ribosomal protein. They are the main source of Ub and the only source of extension ribosomal protein. Although important roles of the Ub-26S proteasome system in various biological processes have been well established, direct evidence for the role ofUEPgenes in plant defense is rarely reported. In this study, we cloned a Ub-S27a-typeUEPgene (NbUEP1) fromNicotiana benthamianaand demonstrated its function in cell death and disease resistance. Virus-induced gene silencing ofNbUEP1led to intensive cell death, culminating in whole-seedling withering. Transient RNA interference (RNAi) ofNbUEP1caused strong cell death in infiltrated areas, while stableNbUEP1-RNAi tobacco plants constitutively formed necrotic lesions in leaves.NbUEP1-RNAi plants exhibited increased resistance to the oomycetePythium aphanidermatumand virusesTobacco mosaic virusandCucumber mosaic viruswhile displaying decreased resistance to the nematodeMeloidogyne incognitacompared with non-RNAi control plants. Transcription profiling analysis indicated that jasmonate and ethylene pathways, lipid metabolism, copper amine oxidase-mediated active species generation, glycine-rich proteins, vacuolar processing enzyme- and RD21-mediated cell death and defense regulation, and autophagy might be associated withNbUEP1-mediated cell death and resistance. Our results provided evidence for the important roles of plant UEPs in modulating plant cell death and disease resistance.