Germ cell transplantation in goats
Germ cell transplantation in goats
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DOI：
10.1002/mrd.10205
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发表时间：
2003-04-01
影响因子：
2.5
通讯作者：
Dobrinski, I
中科院分区：
文献类型：
--
作者：
Honaramooz, A;Behboodi, E;Dobrinski, I
精原干细胞移植为研究精子发生和操纵雄性生殖系提供了一种独特的方法。这项技术也可能为目前生产转基因家畜的低效方法提供另一种选择。我们最近在猪身上建立了精原移植技术，最初是在实验啮齿动物身上开发的，这项研究的目的是将该技术推广到山羊。将分离的供体睾丸细胞通过插入睾丸网状结构的超声引导导管注入麻醉受体山羊的生精小管。供体细胞是通过酶消化新鲜采集的未成熟山羊的睾丸(来自受体的对侧睾丸或来自不相关的供体)获得的。在移植之前，睾丸细胞被标记了荧光标记，以便在移植后进行识别。移植后，每隔3周至12周，受者睾丸检查标记供体细胞的存在和定位。标记的供体细胞见于所有睾丸的生精小管，占受检小管的10-35%。受者睾丸的组织学检查没有发现明显的组织损伤，除了穿刺点有有限的纤维性改变。同样，对移植没有可检测到的局部或全身免疫反应的迹象。这些结果表明，在未成熟的雄性山羊身上进行生殖细胞移植在技术上是可行的，供体来源的细胞在受体睾丸中保留至少三个月并一直持续到青春期。这项研究是关于山羊生殖细胞移植的第一份报告。(C)2003年Wiley-Liss，Inc.
Transplantation of spermatogonial stem cells provides a unique approach for the study of spermatogenesis and manipulation of the male germ line. This technique may also offer an alternative to the currently inefficient methods of producing transgenic domestic animals. We have recently established the technique of spermatogonial transplantation, originally developed in laboratory rodents, in pigs, and this study was aimed to extend the technique to the goat. Isolated donor testis cells were infused into the seminiferous tubules of anesthetized recipient goats through an ultrasonographically-guided catheter inserted into the rete testis. Donor cells were obtained by enzymatic digestion of freshly collected testes from immature goats (either from the recipients' contralateral testis or from unrelated donors). Prior to transplantation, testis cells were labeled with a fluorescent marker to allow identification after transplantation. Recipient testes were examined for the presence and localization of labeled donor cells at 3-week intervals up to 12 weeks after transplantation. Labeled donor cells were found in the seminiferous tubules of all testes, comprising 10-35% of the examined tubules. Histological examination of the recipient testes did not reveal evident tissue damage, except for limited fibrotic changes at the site of needle insertion. Likewise there were no detectable local or systemic signs of immunologic reactions to the transplantations. These results indicate that germ cell transplantation is technically feasible in immature male goats and that donor-derived cells are retained in the recipient testis for at least three months and through puberty. This study represents the first report of germ cell transplantation in goats. (C) 2003 Wiley-Liss, Inc.